Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody [NB200-117] - Distribution of hBD-2 and hBD-3 in normal oral epithelium. Formalin fixed and parafilm embedded normal human oral tissue was labeled with an ...read more
Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody [NB200-117] - Analysis of beta-Defensin 3 in human skin.
Immunohistochemistry: beta-Defensin 3 Antibody - BSA Free [NB200-117] - Topical application of PALA enhances AMP production in human skin explants. (a) HBD2 protein levels are increased in tissue homogenates of infected ...read more
Immunocytochemistry/ Immunofluorescence reported in scientific literature
Immunohistochemistry 1:10-1:500
Immunohistochemistry-Frozen 1:10-1:500
Immunohistochemistry-Paraffin 1:10-1:500
Western Blot 1:500-1:1000
Theoretical MW
7.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB200-117 in the following applications:
Human and rat reactivity reported in (PMID: 21369366).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for beta-Defensin 3 Antibody - BSA Free
BD14
BD3
BD-3
beta-defensin 103
betaDefensin 3
beta-Defensin 3
DEFB103
DEFB103A
DEFB103B
DEFB3
DEFB-3
DEFB3DEFB103A
Defensin, beta 103
defensin, beta 103B
Defensin-like protein
HBD-3
HBD3HBP3
Background
A novel, nonhemolytic antimicrobial peptide (human beta-defensin-3, hBD-3) was isolated from human lesional psoriatic scales and clones from keratinocytes. hBD-3 demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes including multiresistant S. aureus and vancomyosin-resistant Entoerococcus faecium. Keratinocytes and airway epithelial cells are cellular sources of hBD-3. Tumor necrosis factor alpha and contact with bacteria were found to induce hBD-3 mRNA expression. hBD-3, therefore, might be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung, such as cystic fibrosis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Muller C. Quantitative studies of the differential expression of effector molecules of the innate immune system in between oral mucosa and extraoral skin. Thesis: http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20140205-1128930-0-1 2014-02-05 (IHC-P, Human)
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Product General Protocols
View specific protocols for beta-Defensin 3 Antibody (NB200-117):
FAQs for beta-Defensin 3 Antibody (NB200-117). (Showing 1 - 1 of 1 FAQs).
Are there by chance any WB images available for NB200-117 that haven’t been posted yet? If not, do you happen to know what band size(s) were actually detected? We have a customer who is concerned because he has encountered several products that are detecting around 15-17 kDa as opposed to the expected mw of ~8 kDa.
We unfortunately do not have any images available for this product in western blot. I am sorry for any inconvenience. Since western blot is listed on our datasheet, we will of course fully guarantee the application. Typically a band is seen around 10kDa. Depending on the molecular weight marker, I would expect that there might be some difference seen at such a low molecular weight. 15-17kDa does seem a bit too high. I noticed that the protein does have several disulfide binding points. I would make sure your customer is properly boiling their samples (we recommend 10 minutes at 95C) and that they are adding at least 5% reducing agent to their sample buffer. We use BME typically, but DTT is also good.
Beta-defensin-3: I may be small but I'm powerful! Beta-defensin 3 is a novel, non-hemolytic antimicrobial cationic peptide originally isolated from human lesional psoriatic scales and keratinocyte clones. It is a very small (2-6 kD) yet potent salt-insensitive broad spectrum antimicrobial that ta... Read full blog post.
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![Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody [NB200-117] - Distribution of hBD-2 and hBD-3 in normal oral epithelium. Formalin fixed and parafilm embedded normal human oral tissue was labeled with an anti-hBD-2 specific primary antibody, and detected with an AF488 conjugated secondary antibody. hBD-3 was labeled with a rabbit anti-hBD-3 (Novus Biologicals Inc., Littleton, CO, USA), specific primary antibody and detected using an AF594 (purple) conjugated donkey anti-rabbit (Invitrogen). Note green fluorescence detection of hBD-2 localized to the stratum spinosum and stratum granulosum, while pink/red fluorescence detects hBD-3 exclusively to the stratum basale. hBD-1, not shown, is localized to the same regions as hBD-2. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2012.00294/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Defensin-3-Antibody-Immunohistochemistry-Paraffin-NB200-117-img0002.jpg "Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody [NB200-117] - Distribution of hBD-2 and hBD-3 in normal oral epithelium. Formalin fixed and parafilm embedded normal human oral tissue was labeled with an anti-hBD-2 specific primary antibody, and detected with an AF488 conjugated secondary antibody. hBD-3 was labeled with a rabbit anti-hBD-3 (Novus Biologicals Inc., Littleton, CO, USA), specific primary antibody and detected using an AF594 (purple) conjugated donkey anti-rabbit (Invitrogen). Note green fluorescence detection of hBD-2 localized to the stratum spinosum and stratum granulosum, while pink/red fluorescence detects hBD-3 exclusively to the stratum basale. hBD-1, not shown, is localized to the same regions as hBD-2. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2012.00294/abstract) licensed under a CC-BY license.")
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![Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody [NB200-117] - Analysis of beta-Defensin 3 in human skin.](http://images.novusbio.com/fullsize/Defensin-beta-3-Antibody-Immunohistochemistry-Paraffin-NB200-117-img0001.jpg "Immunohistochemistry-Paraffin: beta-Defensin 3 Antibody [NB200-117] - Analysis of beta-Defensin 3 in human skin.")
![Immunohistochemistry: beta-Defensin 3 Antibody - BSA Free [NB200-117] - Topical application of PALA enhances AMP production in human skin explants. (a) HBD2 protein levels are increased in tissue homogenates of infected human skin explants by PALA or Neosporin treatment. Human skin biopsies were infected with MRSA for 1 h & then treated with the indicated ointments for 24 hours in triplicate. HBD2 levels were measured in tissue homogenates by ELISA (n = 3–4 donors). Mean ± SD; significance determined by 1-way ANOVA & Bonferroni multiple comparison test; *p 0.05. (d) Immunohistochemistry staining of HBD3 in MRSA infected human skin explants described in (a). Representative image of 4 donors. (e) LL-37 protein levels are increased in tissue homogenates of infected human skin explants by PALA treatment. LL-37 levels were measured in tissue homogenates described in (a) by ELISA (n = 4–5 donors). Mean ± SD; significance determined by 1-way ANOVA & Bonferroni multiple comparison test; *p < 0.05. (f) Immunohistochemistry staining of LL-37 in MRSA infected human skin explants described in (a). Representative image of 6 donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29880914), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-117_rabbit-polyclonal-beta-defensin-3-antibody-310202416212331.jpg "Immunohistochemistry: beta-Defensin 3 Antibody - BSA Free [NB200-117] - Topical application of PALA enhances AMP production in human skin explants. (a) HBD2 protein levels are increased in tissue homogenates of infected human skin explants by PALA or Neosporin treatment. Human skin biopsies were infected with MRSA for 1 h & then treated with the indicated ointments for 24 hours in triplicate. HBD2 levels were measured in tissue homogenates by ELISA (n = 3–4 donors). Mean ± SD; significance determined by 1-way ANOVA & Bonferroni multiple comparison test; *p 0.05. (d) Immunohistochemistry staining of HBD3 in MRSA infected human skin explants described in (a). Representative image of 4 donors. (e) LL-37 protein levels are increased in tissue homogenates of infected human skin explants by PALA treatment. LL-37 levels were measured in tissue homogenates described in (a) by ELISA (n = 4–5 donors). Mean ± SD; significance determined by 1-way ANOVA & Bonferroni multiple comparison test; *p < 0.05. (f) Immunohistochemistry staining of LL-37 in MRSA infected human skin explants described in (a). Representative image of 6 donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29880914), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")