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beta-Defensin 3 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Rt, Po, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB200-117AF350
Alexa Fluor 405
NB200-117AF405
Alexa Fluor 488
NB200-117AF488
Alexa Fluor 532
NB200-117AF532
Alexa Fluor 594
NB200-117AF594
Alexa Fluor 647
NB200-117AF647
Alexa Fluor 700
NB200-117AF700
Alexa Fluor 750
NB200-117AF750
Allophycocyanin
NB200-117APC
Biotin
NB200-117B
DyLight 350
NB200-117UV
DyLight 405
NB200-117V
DyLight 488
NB200-117G
DyLight 550
NB200-117R
DyLight 594
NB200-117DL594
DyLight 650
NB200-117C
DyLight 680
NB200-117FR
DyLight 755
NB200-117IR
FITC
NB200-117F
HRP
NB200-117H
Janelia Fluor 525
NB200-117JF525
Janelia Fluor 549
NB200-117JF549
Janelia Fluor 646
NB200-117JF646
Janelia Fluor 669
NB200-117JF669
PE
NB200-117PE
PerCP
NB200-117PCP
Unconjugated
NB200-117
mFluor Violet 450 SE
NB200-117MFV450
mFluor Violet 500 SE
NB200-117MFV500
mFluor Violet 610 SE
NB200-117MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB200-117
BSA Free
NB200-117SS
BSA Free
NB200-117
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beta-Defensin 3 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
A synthetic peptide made to an internal portion of Defensin beta 3 (between amino acids 10-60) [UniProt P81534]
Predicted Species
Porcine (90%), Canine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DEFB103B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human and rat reactivity reported in (PMID: 21369366).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for beta-Defensin 3 Antibody [CoraFluor™ 1]

  • BD14
  • BD3
  • BD-3
  • beta-defensin 103
  • betaDefensin 3
  • beta-Defensin 3
  • DEFB103
  • DEFB103A
  • DEFB103B
  • DEFB3
  • DEFB-3
  • DEFB3DEFB103A
  • Defensin, beta 103
  • defensin, beta 103B
  • Defensin-like protein
  • HBD-3
  • HBD3HBP3

Background

Defensin beta 3 is a novel, nonhemolytic antimicrobial peptide (human beta-defensin-3, hBD-3) was isolated from human lesional psoriatic scales and clones from keratinocytes. hBD-3 demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes including multiresistant S. aureus and vancomyosin-resistant Entoerococcus faecium. Keratinocytes and airway epithelial cells are cellular sources of hBD-3. Tumor necrosis factor alpha and contact with bacteria were found to induce hBD-3 mRNA expression. hBD-3, therefore, might be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung, such as cystic fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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beta-Defensin 3
Antibody [CoraFluor&# ...
NB200-117CL1
beta-Defensin 3 Antibody [CoraFluor™ 1]
Species: Hu, Rt, Po, Ca
Applications: WB, ELISA, ICC/IF, IHC

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on beta-Defensin 3.

Check out the latest blog posts on beta-Defensin 3.
Beta-defensin-3: I may be small but I'm powerful!
Beta-defensin 3 is a novel, non-hemolytic antimicrobial cationic peptide originally isolated from human lesional psoriatic scales and keratinocyte clones. It is a very small (2-6 kD) yet potent salt-insensitive broad spectrum antimicrobial that ta...  Read full blog post.
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Bioinformatics

Gene Symbol DEFB103B