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BDNF Antibody (584421) [Unconjugated] - Pro form

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BDNF was detected in immersion fixed paraffin-embedded sections of hippocampus using Mouse Anti-Human Pro BDNF Monoclonal Antibody (Catalog # mab3175) at 15 µg/ml overnight at 4 degrees Celsius followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
BDNF was detected in immersion fixed paraffin-embedded sections of hippocampus using Mouse Anti-Human Pro BDNF Monoclonal Antibody (Catalog # mab3175) at 15 µg/ml overnight at 4 degrees Celsius followed by incubation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
584421
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3175-SP
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BDNF Antibody (584421) [Unconjugated] - Pro form Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Pro-BDNF
Ala19-Arg247
Accession # P23560
Specificity
Detects human Pro-BDNF in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) BDNF (mature), rh beta -NGF, rhNT-3, rhNT-4, rhPro-NT-3 or rhPro-NT-4 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BDNF
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BDNF Antibody (584421) [Unconjugated] - Pro form

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • Neurotrophin

Background

BDNF is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of neuronal subpopulations in the central and peripheral nervous systems. BDNF functions through interactions with the TrkB receptor tyrosine kinase and the low affinity neurotrophin receptor, p75 (NTR). The human BDNF cDNA encodes 247 amino acids (aa). Cleavage of an 18 aa signal sequence produces an approximately 35 kDa Pro-BDNF form. The N-terminal pro region of BDNF is removed by tPA and furin to release biologically active, 14 kDa BDNF. The propeptides of human and mouse BDNF share 93% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BDNF
Uniprot