Home » Aminopeptidase N/CD13 » Aminopeptidase N/CD13 Antibodies » Aminopeptidase N/CD13 Antibody [Unconjugated]

Aminopeptidase N/CD13 Antibody [Unconjugated]

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Western blot shows lysates of human kidney tissue and human prostate tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3815) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human kidney tissue and human prostate tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
U937 human histiocytic lymphoma cell line was stained with Sheep Anti-Human Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3815, filled histogram) or isotype control antibody (Catalog # ...read more
Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3815) at 0.3 ...read more
Simple Western lane view shows lysates of human small intestine tissue and human prostate tissue, loaded at 0.5 mg/mL. A specific band was detected for Aminopeptidase N/CD13 at approximately 177-198 kDa (as ...read more
Western blot shows lysates of U937 human histiocytic lymphoma cell line and human APN knockout U937 human histiocytic lymphoma cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Aminopeptidase ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, IP, CyTOF-ready, KO
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3815
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Catalog# & Formulation Size Price
Carrier Free
AF3815-SP
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Aminopeptidase N/CD13 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase N/CD13
Lys69-Lys967
Accession # P15144
Specificity
Detects human Aminopeptidase N/CD13 in the direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse APN is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ANPEP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 0.3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Knockout Validated 0.5 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF3815 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase N/CD13 Antibody [Unconjugated]

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Background

The human ANPEP gene encodes aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).

  1. Olsen, J. et al. (1988) FEBS Lett. 238:307.
  2. Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
  3. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aminopeptidase N/CD13 Antibody (AF3815)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, IHC-Fr.


Filter By Application
IHC
(2)
IHC-Fr
(1)
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Human
(2)
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Showing Publications 1 - 5 of 5.
Publications using AF3815 Applications Species
Yongmei Liu, Danying Chen, Yuanyuan Wang, Xinglin Li, Yaruo Qiu, Mei Zheng et al. Characterization of CCoV-HuPn-2018 spike protein-mediated viral entry J Virol 9/28/2023 [PMID: 37676001]
Minyong Chen, Diego M. Assis, Matthieu Benet, Colleen M. McClung, Elizabeth A. Gordon, Shourjo Ghose et al. Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer Communications Biology 1/13/2023 [PMID: 36639722]
Lisa L Kirkemo, Susanna K Elledge, Jiuling Yang, James R Byrnes, Jeff E Glasgow, Robert Blelloch et al. Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells eLife 3/8/2022 [PMID: 35257663]
Bertram S, Dijkman R, Habjan M, Heurich A, Gierer S, Glowacka I, Welsch K, Winkler M, Schneider H, Hofmann-Winkler H, Thiel V, Pohlmann S TMPRSS2 activates the human coronavirus 229E for cathepsin-independent host cell entry and is expressed in viral target cells in the respiratory epithelium. J Virol, 2013-03-27;87(11):6150-60. 2013-03-27 [PMID: 23536651] (IHC, IHC-Fr, Human) IHC, IHC-Fr Human
Dijkman R, Jebbink M, Koekkoek S, Deijs M, Jonsdottir H, Molenkamp R, Ieven M, Goossens H, Thiel V, van der Hoek L Isolation and characterization of current human coronavirus strains in primary human epithelial cell cultures reveal differences in target cell tropism. J Virol, 2013-02-20;87(11):6081-90. 2013-02-20 [PMID: 23427150] (IHC, Human) IHC Human

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Bioinformatics

Gene Symbol ANPEP
Uniprot