![Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-Western-Blot-NBP1-32462-img0019.jpg "Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Ze, Bv, Ch, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Alpha Actinin 2. The exact sequence is proprietary. |
| Localization | Cytoplasm, myofibril, sarcomere, Z-disk |
| Predicted Species | Bovine (99%), Xenopus (94%), Chicken (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ACTN2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 104 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publications using NBP1-32462 | Applications | Species |
|---|---|---|
| Meyers TA, Heitzman JA, Krebsbach AM et al. Acute AT1R blockade prevents isoproterenol-induced injury in mdx hearts. J. Mol. Cell. Cardiol. 2019-01-19 [PMID: 30664850] (ICC/IF, Mouse) | ICC/IF | Mouse |
| Meyers TA Preclinical Models of Dystrophic Cardiomyopathy and Therapies for the Dystrophic Heart Thesis (IF/IHC, Mouse) | IF/IHC | Mouse |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ACTN2 |
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse muscle. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32462-img0022.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse muscle. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat muscle. alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32462-img0015.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat muscle. alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. Alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32462-img0016.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. Alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat heart, alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32462-img0017.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat heart, alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32462-img0021.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-91020242014630.jpg "Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Blue: Hoechst 33342 staining.")
![Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-910202420115081.jpg "Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-91020242021988.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-91020242063318.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-91020242021916.jpg "Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-91020242014634.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-910202420122060.jpg "Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-91020242063313.jpg "Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-910202420122056.jpg "Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-910202419593526.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-910202420115037.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-91020242021993.jpg "Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")