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XYLT2 Antibody

Images

 
Western Blot: XYLT2 Antibody [NBP1-33208] - Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: XYLT2 Antibody [NBP1-33208] - Methanol-fixed HeLa, using Xylosyltransferase II antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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XYLT2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Xylosyltransferase II. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane, Single-pass type II membrane protein, Golgi apparatus membrane, Single-pass type II membrane protein
Predicted Species
Mouse (98%), Rat (98%), Chicken (96%), Canine (98%), Bovine (95%), Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XYLT2
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (84%), Japanese Medaka (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for XYLT2 Antibody

  • EC 2.4.2.26
  • Peptide O-xylosyltransferase 1
  • PXYLT2
  • XT2UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase
  • xylosyltransferase 2
  • xylosyltransferase IIXT-IIprotein xylosyltransferase 2
  • xylT-II

Background

The protein encoded by the XYLT2 gene is an isoform of xylosyltransferase, which belongs to a family ofglycosyltransferases. This enzyme transfers xylose from UDP-xylose to specific serine residues of the core protein andinitiates the biosynthesis of glycosaminoglycan chains in proteoglycans including chondroitin sulfate, heparansulfate, heparin and dermatan sulfate. The enzyme activity, which is increased in scleroderma patients, is adiagnostic marker for the determination of sclerotic activity in systemic sclerosis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol XYLT2