Home » Collectrin/TMEM27 » Collectrin Antibodies » Collectrin/TMEM27 Antibody [Unconjugated]

Collectrin/TMEM27 Antibody [Unconjugated]

 (1 Publication)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collectrin at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) followed by HRP-conjugated ...read more
Collectrin was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) at 1.7 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Collectrin at approximately 69 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Collectrin ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4965
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4965-SP
Novus Biologicals offers 100% Guarantee

Collectrin/TMEM27 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Collectrin
Glu15-Ile144
Accession # Q9ESG4
Specificity
Detects mouse Collectrin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Collectrin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TMEM27
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF4965 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Collectrin/TMEM27 Antibody [Unconjugated]

  • Collectrin
  • kidney-specific membrane protein
  • NX17
  • NX-17
  • TMEM27
  • transmembrane protein 27NX170610008J07Rik

Background

Collectrin (also Transmembrane protein 27) is a 37‑43 kDa member of the MEM27 family of proteins. It is expressed on pancreatic beta -cells where it regulates insulin secretion, and on renal tubular epithelium where it mediates amino acid transport. Mature mouse Collectrin is a 208 amino acid (aa) type I transmembrane glycoptotein. It contains a 127 aa extracellular region (aa 15‑141) and a 60 aa cytoplasmic domain (aa 163‑222). Collectrin dimerizes on the cell surface, and undergoes proteolytic cleavage to generate a 26 kDa soluble fragment. Over aa 15‑144, mouse Collectrin shares 92% and 86% aa identity with rat and human Collectrin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

ACE-2 Antibody
[Unconjugated]
AF933
Simple Western ACE-2 Antibody [Unconjugated]
Immunohistochemistry ACE-2 Antibody [Unconjugated]
Immunohistochemistry ACE-2 Antibody [Unconjugated]
(1 Review)
(297 Publications)
Species: Ha, Hu, Mu, Rt
Applications: Block, Flow, IHC, IP, Simple Western, WB
HNF1 Antibody
NBP1-33596
Western Blot HNF1 Antibody
Western Blot HNF1 Antibody
Immunoprecipitation HNF1 Antibody
(6 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, PAGE, WB
SLC6A19 Antibody
NBP2-48784
Immunocytochemistry/ Immunofluorescence SLC6A19 Antibody
Immunohistochemistry-Paraffin SLC6A19 Antibody
Immunohistochemistry-Paraffin SLC6A19 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
ACE/CD143 Antibody
[Unconjugated]
AF1513
Immunohistochemistry ACE/CD143 Antibody [Unconjugated]
Western Blot ACE/CD143 Antibody [Unconjugated]
Simple Western ACE/CD143 Antibody [Unconjugated]
(1 Review)
(7 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(25 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
AGTR-1 Antibody -
BSA Free
NBP1-77078
Western Blot AGTR-1 Antibody - BSA Free
Immunohistochemistry AGTR-1 Antibody - BSA Free
Immunohistochemistry AGTR-1 Antibody - BSA Free
(10 Publications)
Species: Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Acetyl-coenzyme A
transporter 1 Antibod ...
H00009197-M07
Western Blot Acetyl-coenzyme A transporter 1 Antibody (3A4)
Western Blot Acetyl-coenzyme A transporter 1 Antibody (3A4)
Western Blot Acetyl-coenzyme A transporter 1 Antibody (3A4)
(2 Publications)
Species: Ha, Hu, Mu, Rt
Applications: ELISA, ICC/IF, KD, WB
ATM Antibody
NB100-104
Flow Cytometry ATM Antibody
Immunohistochemistry-Paraffin ATM Antibody
Flow Cytometry ATM Antibody
(2 Reviews)
(100 Publications)
Species: Ca, Hu, Ma, Mu, Rt
Applications: ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, WB
TCF-2/HNF-1 beta
Antibody
NBP1-89680
Immunocytochemistry/ Immunofluorescence TCF-2/HNF-1 beta Antibody
Immunohistochemistry-Paraffin TCF-2/HNF-1 beta Antibody
Immunohistochemistry-Paraffin TCF-2/HNF-1 beta Antibody
(1 Review)
(7 Publications)
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, Simple Western, WB
Snapin Antibody
(809116)
MAB7795
Western Blot Snapin Antibody (809116)
Species: Hu, Mu
Applications: WB
SLC6A18 Antibody
NBP1-82024
Immunohistochemistry-Paraffin SLC6A18 Antibody
Immunohistochemistry-Paraffin SLC6A18 Antibody
Immunohistochemistry-Paraffin SLC6A18 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
Serpin
A8/Angiotensinogen An ...
NBP1-30027
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
Western Blot Serpin A8/Angiotensinogen Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
(12 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
FXYD2 Antibody
(1C3-B3)
H00000486-M01
Western Blot FXYD2 Antibody (1C3-B3)
Sandwich ELISA Capture FXYD2 Antibody (1C3-B3)
Immunohistochemistry-Paraffin FXYD2 Antibody (1C3-B3)
(2 Publications)
Species: Hu
Applications: ELISA, IHC, IHC-P, ELISA(Cap), S-ELISA, WB
ZnT-8/SLC30A8
Antibody (815039) [Un ...
MAB7936
Immunohistochemistry ZnT-8/SLC30A8 Antibody (815039) [Unconjugated]
Intracellular Staining by Flow Cytometry ZnT-8/SLC30A8 Antibody (815039) [Unconjugated]
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow
DNER Antibody
[Unconjugated]
AF2254
Immunohistochemistry DNER Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence DNER Antibody [Unconjugated]
(12 Publications)
Species: Mu
Applications: IHC, WB
Neurogenin-3
Antibody [Unconjugate
AF3444
Western Blot Neurogenin-3 Antibody [Unconjugated]
Immunohistochemistry Neurogenin-3 Antibody [Unconjugated]
(19 Publications)
Species: Hu
Applications: IHC, WB

Publications for Collectrin/TMEM27 Antibody (AF4965)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for Collectrin/TMEM27 Antibody (AF4965) (0)

There are no reviews for Collectrin/TMEM27 Antibody (AF4965). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Collectrin/TMEM27 Antibody (AF4965) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Collectrin/TMEM27 Products

Blogs on Collectrin/TMEM27

There are no specific blogs for Collectrin/TMEM27, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Collectrin/TMEM27 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol TMEM27
Uniprot