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Vinculin Antibody (VCL/2575) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, MA
Clone
VCL/2575
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-79942AF350
Alexa Fluor 405
NBP2-79942AF405
Alexa Fluor 488
NBP2-79942AF488
Alexa Fluor 532
NBP2-79942AF532
Alexa Fluor 594
NBP2-79942AF594
Alexa Fluor 647
NBP2-79942AF647
Alexa Fluor 700
NBP2-79942AF700
Alexa Fluor 750
NBP2-79942AF750
Allophycocyanin
NBP2-79942APC
Biotin
NBP2-79942B
DyLight 350
NBP2-79942UV
DyLight 405
NBP2-79942V
DyLight 488
NBP2-79942G
DyLight 550
NBP2-79942R
DyLight 594
NBP2-79942DL594
DyLight 650
NBP2-79942C
DyLight 680
NBP2-79942FR
DyLight 755
NBP2-79942IR
FITC
NBP2-79942F
HRP
NBP2-79942H
Janelia Fluor 525
NBP2-79942JF525
Janelia Fluor 549
NBP2-79942JF549
Janelia Fluor 646
NBP2-79942JF646
Janelia Fluor 669
NBP2-79942JF669
PE
NBP2-79942PE
PerCP
NBP2-79942PCP
Unconjugated
NBP2-79808-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-79808-100ug
Azide and BSA Free
NBP2-79942
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Vinculin Antibody (VCL/2575) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary) (Uniprot: P18206)
Localization
Cytoplasmic
Marker
Marker of Age-related Macular Degeneration
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VCL
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Vinculin Antibody (VCL/2575) [CoraFluor™ 1]

  • CMD1W
  • CMH15
  • metavinculin
  • MVCL
  • VCL
  • Vinculin

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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FAQs for Vinculin Antibody (NBP2-79942CL1). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VCL