![Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] diluted at 1:500.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Western-Blot-NBP2-15723-img0009.jpg "Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] diluted at 1:500.")
| Reactivity | Hu, Mu, Rt, Bv, Rb, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Catenin alpha 1. The exact sequence is proprietary. |
| Localization | Cytoplasm; cytoskeleton; Cell junction; adherens junction; Cell membrane |
| Predicted Species | Rhesus Macaque (99%), Bovine (97%), Rabbit (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CTNNA1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 100 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol (pH7) |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
![Immunohistochemistry VE-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/VECadherin_AF1002_Immunohistochemistry_22098.jpg)
![Western Blot VE-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/VECadherin_AF1002_Western_Blot_17319.jpg)
![Simple Western VE-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/VECadherin_AF1002_Western_Blot_17475.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Catenin alpha 1 Antibody (NBP2-15723)Find related products by research area.
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![Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse intestine. Green: alpha Catenin antibody [N1N3] diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15723-img0011.jpg "Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse intestine. Green: alpha Catenin antibody [N1N3] diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.")
![Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse intestine. alpha 1 Catenin antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15723-img0007.jpg "Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse intestine. alpha 1 Catenin antibody [N1N3] dilution: 1:500.")
![Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse prostate. alpha 1 Catenin antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15723-img0008.jpg "Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse prostate. alpha 1 Catenin antibody [N1N3] dilution: 1:500.")
![Immunoprecipitation: Catenin alpha 1 Antibody [NBP2-15723] - IP samples: HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of CTNNA1 protein by 4 ug alpha 1 Catenin antibody [N1N3] 5 % SDS-PAGE The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Immunoprecipitation-NBP2-15723-img0006.jpg "Immunoprecipitation: Catenin alpha 1 Antibody [NBP2-15723] - IP samples: HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of CTNNA1 protein by 4 ug alpha 1 Catenin antibody [N1N3] 5 % SDS-PAGE The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -Sample (30 ug of whole cell lysate) A: NIH-3T3 5% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/antibody/nbp2-15723_rabbit-polyclonal-catenin-alpha-1-antibody-western-blot-662023103211..jpg "Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -Sample (30 ug of whole cell lysate) A: NIH-3T3 5% SDS PAGE diluted at 1:500")
![Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -Sample (30 ug of whole cell lysate), A: 293T, B: A431, C: HeLa 5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/antibody/nbp2-15723_rabbit-polyclonal-catenin-alpha-1-antibody-western-blot-662023103415..jpg "Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -Sample (30 ug of whole cell lysate), A: 293T, B: A431, C: HeLa 5% SDS PAGE diluted at 1:1000")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")