N-Cadherin Antibody (13A9)

N-Cadherin, also referred to as Neural Cadherin (NCAD) or Cadherin-2 (CHD2), is a 130 kDa protein that is a member of the calcium-dependent adhesion molecule family of classical (type I) cadherins (1-4). Under the CDH2 gene, human N-cadherin is synthesized as a 906 amino acid protein with a theoretical molecular weight of 99.8 kDa (5). The N-cadherin protein structure is similar to other classical type I cadherins including epithelial (E-) cadherin and placental (P-) cadherin (1,2). N-cadherin consists of a 25 amino acid (aa) N-terminal signal peptide and 134 aa pro-peptide, a 565 aa extracellular domain (ECD) with five cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (1-3,5). The ECD of N-cadherin monomers is responsible for homotypic binding through either cis or trans adhesion (2,3).

N-cadherin is expressed on multiple cell types but is most highly expressed by mesenchymal cells and neural tissue (2). Functionally, N-cadherin has a number of roles including maintaining structural integrity and adhesion, cell signaling, and formation of neuronal synapses and the vascular wall (2). The cytoplasmic tail interacts with beta-catenin which then binds with alpha-catenin, forming the cadherin-catenin adhesion complex, an important component of adhesions junctions (1-3). Given its role in adhesion, N-cadherin serves as an indicator of epithelial-to-mesenchymal transition (EMT) (1-4). The loss of E-cadherin during EMT corresponds with an increase in N-cadherin expression (1-4). This "cadherin-switch" is associated with increased migratory and invasive behavior observed in tumor progress (1-4). Proteases including activity of a disintegrin and metalloprotease 10 (ADAM10), matrix metalloproteinases (MMPs), caspase 3, presenilin, and calpain can cleave N-cadherin as a mechanism for regulating Wnt/beta-catenin signaling and inducing oncogenic signals (3,4). In addition to its expression in solid tumors, N-cadherin has been indicated in hematological disorders such as leukemia and multiple myeloma (1). N-cadherin antagonists are currently being studied as potential therapeutics for a variety of cancer studies (1-2).

References

1. Mrozik, K. M., Blaschuk, O. W., Cheong, C. M., Zannettino, A., & Vandyke, K. (2018). N-cadherin in cancer metastasis, its emerging role in haematological malignancies and potential as a therapeutic target in cancer. BMC Cancer. https://doi.org/10.1186/s12885-018-4845-0

2. Loh, C. Y., Chai, J. Y., Tang, T. F., Wong, W. F., Sethi, G., Shanmugam, M. K., Chong, P. P., & Looi, C. Y. (2019). The E-Cadherin and N-Cadherin Switch in Epithelial-to-Mesenchymal Transition: Signaling, Therapeutic Implications, and Challenges. Cells. https://doi.org/10.3390/cells8101118

3. Derycke, L. D., & Bracke, M. E. (2004). N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signalling. The International Journal of Developmental Biology. https://doi.org/10.1387/ijdb.041793ld

4. Yu, W., Yang, L., Li, T., & Zhang, Y. (2019). Cadherin Signaling in Cancer: Its Functions and Role as a Therapeutic Target. Frontiers in Oncology. https://doi.org/10.3389/fonc.2019.00989

5. Unitprot (P1903)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 10 applications: FLOW, ICC, ICC/IF, IF/IHC, IHC, IHC-P, IP, Simple Western, WB, Western Blot.

Showing Publications 1 - 10 of 41. Show All 41 Publications. Collapse Publications.

Publications using NBP1-48309	Applications	Species
Belghasem M, Yin W, Lotfollahzadeh S et al. Tryptophan Metabolites Target Transmembrane and Immunoglobulin Domain-Containing 1 Signaling to Augment Renal Tubular Injury The American journal of pathology 2023-10-01 [PMID: 37676196] (WB, Mouse)	WB	Mouse
?wierczewska M, Sterzy?ska K, Ruci?ski M et al. The response and resistance to drugs in ovarian cancer cell lines in 2D monolayers and 3D spheroids Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-07-11 [PMID: 37442067] (IHC, Human)	IHC	Human
Lin YK, Zhang F, Lei WJ et al. Amnion-derived serum amyloid A1 participates in sterile inflammation of fetal membranes at parturition Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2023-03-06 [PMID: 36879064]
Huang M, Wang C, Yao Y et al. Mebendazole-Induced Blood-Testis Barrier Injury in Mice Testes by Disrupting Microtubules in Addition to Triggering Programmed Cell Death International journal of molecular sciences 2022-04-11 [PMID: 35457043]
Karim S, Burzangi AS, Ahmad A Et al. PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer Int J Mol Sci 2022-02-26 [PMID: 35216429] (WB, Human) Details: Citation using the HRP version of this antibody.	WB	Human
Padder RA, Bhat ZI, Ahmad Z et al. DRP1 Promotes BRAF(V600E)-Driven Tumor Progression and Metabolic Reprogramming in Colorectal Cancer Frontiers in Oncology 2021-03-02 [PMID: 33738242] (Western Blot)	Western Blot
Mohammed S, Torres Luquis O, Walls E, Lloyd F. Lymph-Circulating Tumor Cells show distinct properties to Blood-Circulating Tumor Cells and constitute extraordinary efficient metastatic precursors Mol Oncol 2019-04-27 [PMID: 31026363] (ICC/IF)	ICC/IF
Matta R, Yousafzai MS, Murrell M, Gonzalez AL Endothelial cell secreted metalloproteinase-2 enhances neural stem cell N-cadherin expression, clustering, and migration FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-02-01 [PMID: 33417253]
SAenz-de-Santa-MarIa I, Celada L, San JosE MartInez A et al. Blockage of Squamous Cancer Cell Collective Invasion by FAK Inhibition Is Released by CAFs and MMP-2 Cancers 2020-12-10 [PMID: 33321813] (WB, Human)	WB	Human
Beauchamp P, Jackson C B et al. 3D Co-culture of hiPSC-Derived Cardiomyocytes With Cardiac Fibroblasts Improves Tissue-Like Features of Cardiac Spheroids. Front Mol Biosci 2020-03-03 [PMID: 32118040] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhao, K;Li, X;Chen, X;Zhu, Q;Yin, F;Ruan, Q;Xia, J;Niu, Z; Inhibition of miR-140-3p or miR-155-5p by antagomir treatment sensitize chordoma cells to chemotherapy drug treatment by increasing PTEN expression Eur. J. Pharmacol. 2019-04-10 [PMID: 30980798] (WB, Human)	WB	Human
Hlavac N, VandeVord PJ. Astrocyte Mechano-Activation by High-Rate Overpressure Involves Alterations in Structural and Junctional Proteins Front Neurol 2019-02-22 [PMID: 30853931] (Simple Western, Rat)	Simple Western	Rat
Ren W, Hou J, Yang C et al. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res. 2019-02-08 [PMID: 30736829] (WB, Human)	WB	Human
Chiba Y, He B, Yoshizaki K et al. The transcription factor AmeloD stimulates epithelial cell motility essential for tooth morphology J. Biol. Chem. 2018-11-30 [PMID: 30504223] (IF/IHC, Mouse)	IF/IHC	Mouse
Park S, Kim H, Kim K et al. Sonic hedgehog signalling regulates the self-renewal and proliferation of skin-derived precursor cells in mice Cell Prolif. 2018-08-27 [PMID: 30151845] (ICC/IF, Mouse)	ICC/IF	Mouse
Chen CY, Melo E, Jakob P et al. N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration. Matrix Biol. 2018-03-20 [PMID: 29572155] (IF/IHC)	IF/IHC
Melo E, Oertle P, Trepp C et al. HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model. EBioMedicine 2017-12-13 [PMID: 29269042] (IF/IHC, Human)	IF/IHC	Human
Becker A B, Qian J et al. Heart and Nervous System Pathology in Compound Heterozygous Friedreich Ataxia. J Neuropathol Exp Neurol 2017-01-08 [PMID: 28789479] (IF/IHC, Human)	IF/IHC	Human
Farjood Farhad, Vargis Elizabeth Physical disruption of cell-cell contact induces VEGF expression in RPE cells Physical disruption of cell-cell contact induces VEGF expression in RPE cells. Mol Vis. 2017-07-17 [PMID: 28761317] (ICC, Human) Details: Citation using the DyLight 680 version of this antibody.	ICC	Human
Koeppen AH, Becker AB, Feustel PJ et al. The significance of intercalated discs in the pathogenesis of Friedreich cardiomyopathy. J Neurol Sci 2016-08-15 [PMID: 27423584]
Michelhaugh SK, Guastella AR, Varadarajan K et al. Development of patient-derived xenograft models from a spontaneously immortal low-grade meningioma cell line, KCI-MENG1 J Transl Med. 2015-07-16 [PMID: 26174772] (ICC/IF, Human) Details: N-Cadherin antibody used for Immunocytochemistry on KCI-MENG1 cells or KCI-MENG1-LPSX cells, which are dissociated cells from second generation xenograft mouse tumor) at a dilution of 1:100.	ICC/IF	Human
Chow AK, Ng L, Lam CS et al. The Enhanced Metastatic Potential of Hepatocellular Carcinoma (HCC) Cells with Sorafenib Resistance. PLoS One. 2013-11-11 [PMID: 24244338] (WB, Human)	WB	Human
Maeda M, Johnson E, Mandal SH, Lawson KR, Keim SA, Svoboda RA, Caplan S, Wahl JK 3rd, Wheelock MJ, Johnson KR. Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn). Oncogene25(33):4595-604. 2006-08-03 [PMID: 16786001] (WB, Human)	WB	Human
Jaggi M, Nazemi T, Abrahams NA, Baker JJ, Galich A, Smith LM, Balaji KC. N-cadherin switching occurs in high Gleason grade prostate cancer. Prostate66(2):193-9. 2006-02-01 [PMID: 16173043] (IHC-P, Human)	IHC-P	Human
Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ. N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem278(19):17269-76. 2003-05-09 [PMID: 12604612] (ICC/IF, WB, IP, Human)	ICC/IF, WB, IP	Human
Van Aken EH, Papeleu P, De Potter P, Bruyneel E, Philippe J, Seregard S, Kvanta A, De Laey JJ, Mareel MM. Structure and function of the N-cadherin/catenin complex in retinoblastoma. Invest Ophthalmol Vis Sci43(3):595-602. 2002-03-01 [PMID: 11867572] (IP, ICC/IF, WB, FLOW, Human)	IP, ICC/IF, WB, FLOW	Human
Reyes-Mugica M, Meyerhardt JA, Rzasa J, Rimm DL, Johnson KR, Wheelock MJ, Reale MA. Truncated DCC reduces N-cadherin/catenin expression and calcium-dependent cell adhesion in neuroblastoma cells. Lab Invest81(2):201-10. 2001-02-01 [PMID: 11232642] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Kim JB, Islam S, Kim YJ, Prudoff RS, Sass KM, Wheelock MJ, Johnson KR. N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility. J Cell Biol151(6):1193-206. 2000-12-11 [PMID: 11121435] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Mialhe A, Levacher G, Champelovier P, Martel V, Serres M, Knudsen K, Seigneurin D. Expression of E-, P-, n-cadherins and catenins in human bladder carcinoma cell lines. J Urol164(3 Pt 1):826-35. 2000-09-01 [PMID: 10953163]
Nieman MT, Prudoff RS, Johnson KR, Wheelock MJ. N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression. J Cell Biol;147(3):631-44. 1999-11-01 [PMID: 10545506] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Makrigiannakis A, Coukos G, Christofidou-Solomidou M, Gour BJ, Radice GL, Blaschuk O, Coutifaris C. N-cadherin-mediated human granulosa cell adhesion prevents apoptosis: a role in follicular atresia and luteolysis? . Am J Pathol;154(5):1391-406. 1999-05-01 [PMID: 10329592] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Benson DL, Tanaka H. N-cadherin redistribution during synaptogenesis in hippocampal neurons. J Neurosci18(17):6892-904. 1998-09-01 [PMID: 9712659] (ICC/IF, WB, Rat)	ICC/IF, WB	Rat
Navarro P, Ruco L, Dejana E. Differential localization of VE- and N-cadherins in human endothelial cells: VE-cadherin competes with N-cadherin for junctional localization. J Cell Biol140(6):1475-84. 1998-03-23 [PMID: 9508779] (ICC/IF, Human)	ICC/IF	Human
Peralta Soler A, Knudsen KA, Tecson-Miguel A, McBrearty FX, Han AC, Salazar H. Expression of E-cadherin and N-cadherin in surface epithelial-stromal tumors of the ovary distinguishes mucinous from serous and endometrioid tumors. Hum Pathol28(6):734-9. 1997-06-01 [PMID: 9191009]
Han AC, Peralta-Soler A, Knudsen KA, Wheelock MJ, Johnson KR, Salazar H. Differential expression of N-cadherin in pleural mesotheliomas and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues. Hum Pathol28(6):641-5. 1997-06-01 [PMID: 9190996] (Human)		Human
Hazan RB, Kang L, Whooley BP, Borgen PI. N-cadherin promotes adhesion between invasive breast cancer cells and the stroma. Cell Adhes Commun4(6):399-411. 1997-03-01 [PMID: 9177902]
Islam S, Carey TE, Wolf GT, Wheelock MJ, Johnson KR. Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion. J Cell Biol;135(6 Pt 1):1643-54. 1996-12-01 [PMID: 8978829] (IP, ICC/IF, WB, Human)	IP, ICC/IF, WB	Human
Peralta Soler A, Knudsen KA, JaurMC, Johnson KR, Wheelock MJ, Klein-Szanto AJ, Salazar H. The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas. Hum Pathol26(12):1363-9. 1995-12-01 [PMID: 8522310] (ICC/IF, Human)	ICC/IF	Human
Knudsen KA, Soler AP, Johnson KR, Wheelock MJ. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol;130(1):67-77. 1995-07-01 [PMID: 7790378] (ICC/IF, WB, IP, Human)	ICC/IF, WB, IP	Human
Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem;270(34):20201-6. 1995-08-25 [PMID: 7650039] (WB, Human)	WB	Human
Show All 41 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	ICC	Mouse	07/23/2020	View Summary Application Immunocytochemistry Sample Tested adult mouse neural stem cells Species Mouse Lot C-2
	5 reviewed by: Verified Customer	WB	Human	12/28/2011	View Summary Application Western Blot Sample Tested Human Species Human

Array NBP1-48309

• N-Cadherin Antibodies
• N-Cadherin ELISA Kits
• N-Cadherin Lysate
• N-Cadherin Proteins
• N-Cadherin Proteins and Enzymes
• N-Cadherin ELISA