Novus Biologicals products are now on bio-techne.com

FZR1/CDH1 Antibody

Images

 
Western Blot: FZR1/CDH1 Antibody [NBP2-15840] - Cdh1 decreases upon Abeta treatment in neurons. Representative Western blot image of cdh1 after different time points in control conditions or with Abeta treatment. ...read more
Immunocytochemistry/ Immunofluorescence: FZR1/CDH1 Antibody [NBP2-15840] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FZR1 protein stained by FZR1 antibody diluted at 1:500. Red: Phalloidin, ...read more
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse brain. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: FZR1/CDH1 Antibody [NBP2-15840] - A431 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FZR1/CDH1 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse heart. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Rat heart. FZR1 antibody (GTX111200) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

FZR1/CDH1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human FZR1. The exact sequence is proprietary.
Localization
Isoform 2: Nucleus; Isoform 3: Cytoplasm
Predicted Species
Porcine (97%), Bovine (97%), Rhesus Macaque (100%), Xenopus (90%), Chicken (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FZR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-15840 in the following applications:

Reactivity Notes

Xenopus laevis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for FZR1/CDH1 Antibody

  • CDC20C
  • CDH1
  • Cdh1/Hct1 homolog
  • CDH1CDC20-like 1b
  • fizzy/cell division cycle 20 related 1 (Drosophila)
  • Fzr
  • FZR2
  • FZRCDC20-like protein 1
  • hCDH1
  • HCDHFYR
  • KIAA1242fizzy-related protein homolog

Background

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-86509
Species: Hu
Applications: IHC, IHC-P
NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB100-59828
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP2-03644
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF6000
Species: Hu, Mu
Applications: IHC, Simple Western, WB
NBP2-67381
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
NBP3-21649
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NB100-168
Species: Hu, Mu(-)
Applications: CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-16756
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-82454
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-45603
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AF1329
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
NBP2-20287
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-31361
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-81988
Species: Hu
Applications: IHC, IHC-P
NBP2-21037
Species: Hu
Applications: IHC, IHC-P, WB
AF3264
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP2-15840
Species: Hu, Mu, Rt, Po, Bv, Ch, RM, Xp
Applications: WB, ICC/IF, IHC

Publications for FZR1/CDH1 Antibody (NBP2-15840)(5)

Reviews for FZR1/CDH1 Antibody (NBP2-15840) (0)

There are no reviews for FZR1/CDH1 Antibody (NBP2-15840). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FZR1/CDH1 Antibody (NBP2-15840) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional FZR1/CDH1 Products

Research Areas for FZR1/CDH1 Antibody (NBP2-15840)

Find related products by research area.

Blogs on FZR1/CDH1

There are no specific blogs for FZR1/CDH1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FZR1/CDH1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol FZR1
Uniprot