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Paxillin Antibody [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific diffuse band was detected for Paxillin at approximately 65 to 70 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell ...read more
Paxillin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) at 10 µg/mL for 3 hours ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Paxillin at approximately 70 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4259
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4259-SP
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Paxillin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Paxillin
Asn59-Ser274
Accession # P49023
Specificity
Detects human, mouse, and rat Paxillin in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PXN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4259 in the following applications:

  • ICC
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Paxillin Antibody [Unconjugated]

  • FLJ16691
  • Paxillin
  • PXN

Background

The cytoskeletal adaptor protein Paxillin is found at the interface between actin filaments and the plasma membrane. Paxillin localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation. Increased Paxillin protein levels have also been reported in several malignancies, including renal, lung, and breast carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Paxillin Antibody
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Paxillin Antibody [Unconjugated]
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Publications for Paxillin Antibody (AF4259)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


Filter By Application
ICC
(2)
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Human
(2)
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Showing Publications 1 - 7 of 7.
Publications using AF4259 Applications Species
Saurav Kumar, Amanda Stainer, Julien Dubrulle, Christopher Simpkins, Jonathan A Cooper Cas phosphorylation regulates focal adhesion assembly eLife 2023-07-25 [PMID: 37489578]
Elizabeth M Steenkiste, Jason D Berndt, Carissa Pilling, Christopher Simpkins, Jonathan A Cooper A Cas-BCAR3 co-regulatory circuit controls lamellipodia dynamics eLife 2021-06-25 [PMID: 34169835]
Chen Y, Tristan CA, Lu C et al. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells Nat Methods 5/4/2021 [PMID: 33941937]
Antoniades I, Kyriakou M, Charalambous A, et al. FAK displacement from focal adhesions: a promising strategy to target processes implicated in cancer progression and metastasis Cell communication and signaling : CCS 1/7/2021 [PMID: 33413438]
Stephen E. McGowan, Diann M. McCoy Platelet-derived growth factor receptor-alpha and Ras-related C3 botulinum toxin substrate-1 regulate mechano-responsiveness of lung fibroblasts American Journal of Physiology-Lung Cellular and Molecular Physiology 2017-12-01 [PMID: 28775097]
Etzold A, Galetzka D, Weis E, Bartsch O, Haaf T, Spix C, Itzel T, Schweiger S, Strand D, Strand S, Zechner U CAF-like state in primary skin fibroblasts with constitutional BRCA1 epimutation sheds new light on tumor suppressor deficiency-related changes in healthy tissue. Epigenetics, 2016-03-07;11(2):120-31. 2016-03-07 [PMID: 26949839] (ICC, Human) ICC Human
Ho F, Zhang W, Li Y, Chan B Mechanoresponsive, omni-directional and local matrix-degrading actin protrusions in human mesenchymal stem cells microencapsulated in a 3D collagen matrix. Biomaterials, 2015-03-19;53(0):392-405. 2015-03-19 [PMID: 25890737] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol PXN
Uniprot