Do you have any 100 coda or bigger controls?

Name: Vinculin Antibody (hVIN-1)
Brand: Novus Biologicals
SKU: NB600-1293
Rating: 5 (1 reviews)

Question

Accepted Answer

I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Am, Bv, Ca, Ch, TrSpecies Glossary
Applications	WB, Simple Western, ICC/IF, IHC, Single-Cell Western
Clone	hVIN-1
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Immunogen	Purified human vinculin from uterus.
Localization	Cytoplasm; cytoskeleton. Cell junction; adherens junction; cytoplasmic side. Cytoplasmic face of adhesion plaques.
Marker	Focal Adhesion Marker
Specificity	Specifically labels vinculin at cell-cell and cell-substrate contacts. Shows cross-reactivity with smooth muscle metavinculin.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	VCL
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	Ascites
Preservative	0.09% Sodium Azide
Purity	Unpurified

Publications using NB600-1293	Applications	Species
Hasegawa J, Nagata T, Ihara K et al. Heteroduplex oligonucleotide technology boosts oligonucleotide splice switching activity of morpholino oligomers in a Duchenne muscular dystrophy mouse model Nature Communications 2024-09-26 [PMID: 39327422]
Li H, Huynh TN, Duong MT et al. ACAT1/SOAT1 Blockade Suppresses LPS-Mediated Neuroinflammation by Modulating the Fate of Toll-like Receptor 4 in Microglia International journal of molecular sciences 2023-03-15 [PMID: 36982689] (Western Blot, Mouse)	Western Blot	Mouse
Ventura E, Xie C, Buraschi S et al. Complexity of progranulin mechanisms of action in mesothelioma Journal of experimental & clinical cancer research : CR 2022-12-05 [PMID: 36471440] (Immunocytochemistry/ Immunofluorescence, Human)	Immunocytochemistry/ Immunofluorescence	Human
Costanzo F, Martinez Diez M, Santamaria Nunez G et al. Promoters of ASCL1- and NEUROD1-dependent genes are specific targets of lurbinectedin in SCLC cells EMBO molecular medicine 2022-03-09 [PMID: 35263037] (WB, Human)	WB	Human
Uzureau S, Lecordier L, Uzureau P et Al. APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin Cell Rep 2020-03-17 [PMID: 32187552] (ICC/IF, Human)	ICC/IF	Human
X Zhou, S Wahane, MS Friedl, M Kluge, CC Friedel, K Avrampou, V Zachariou, L Guo, B Zhang, X He, RH Friedel, H Zou Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2 Nat. Neurosci., 2020-03-01;23(3):337-350. 2020-03-01 [PMID: 32112058]
Wu L, Gu Y, Liu L et al. Hierarchical micro/nanofibrous membranes of sustained releasing VEGF for periosteal regeneration Biomaterials 2019-10-18 [PMID: 31655445] (ICC/IF, Human)	ICC/IF	Human
Miao Q, Hill MC, Chen F et al. SOX11 and SOX4 drive the reactivation of an embryonic gene program during murine wound repair Nat Commun [PMID: 31492871] (ICC/IF, IF/IHC, Human)	ICC/IF, IF/IHC	Human
Watanabe K, Yui Y, Sasagawa S et al. Low-dose eribulin reduces lung metastasis of osteosarcoma in vitro and in vivo Oncotarget 2019-01-04 [PMID: 30719211] (ICC/IF, Mouse)	ICC/IF	Mouse
Yadav N, Jaber FL, Sharma Y et al. Efficient reconstitution of hepatic microvasculature by endothelin receptor antagonism in liver sinusoidal endothelial cells. Hum. Gene Ther. 2018-09-28 [PMID: 30266073] (ICC/IF, Mouse)	ICC/IF	Mouse
da Rocha JT, Trapani L, Segatto M et al. Molecular Effects of Diphenyl Diselenide on Cholesterol and Glucose Cell Metabolism. Curr Med Chem. 2013-01-01 [PMID: 23590714]
Chianale, F et al. Diacylglycerol Kinase-alpha Mediates HGF-induced Epithelial Cell Scatter by Regulating Rac Activation Membrane Ruffling. Mol. Biol. Cell. 10.1091/mbc.E07-02-0177. 2007-09-01 [PMID: 17898083]
Wu L, Xu Y, Xi K et al. Regulation of macrophage subtype via injectable micro/nano-structured porous microsphere for reprogramming osteoimmune microenvironment Chemical Engineering Journal 2022-07-01 (ICC/IF, Rat)	ICC/IF	Rat
Li Y, Li C, Liu Q et al. Loss of Acta2 in cardiac fibroblasts does not prevent the myofibroblast differentiation or affect the cardiac repair after myocardial infarction Journal of molecular and cellular cardiology 2022-08-22 [PMID: 36007455] (IHC-Fr, Mouse) Details: IHC-Fr dilution 1:100	IHC-Fr	Mouse
Show All 14 Publications. Collapse Publications.

Vinculin Antibody (hVIN-1)

Species Glossary

Images

Product Details

Order Details

Vinculin Antibody (hVIN-1) Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for Vinculin Antibody (hVIN-1)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Vinculin Antibody (NB600-1293)(14)

Review for Vinculin Antibody (NB600-1293) (1) 51

Summary

Product General Protocols

Video Protocols

FAQs for Vinculin Antibody (NB600-1293). (Showing 1 - 1 of 1 FAQs).

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Additional Vinculin Products

Research Areas for Vinculin Antibody (NB600-1293)

Blogs on Vinculin

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Recent Reviews

Bioinformatics

Gene Symbol	VCL
Entrez	7414(Human) 22330(Mouse) 305679(Rat)
Uniprot	P18206()

	Qi Miao 04/26/2017
Application:	ICC
Species:	Mouse