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VEGFR2/KDR/Flk-1 Antibody [Biotin]

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Humoral response in pre-clinical models. Specific IgG antibodies were detected by ELISA using GST-hVEGF as coating antigen (a, b, d and f). The ability of animal sera antibodies to block VEGF/VEGFR2 interaction was determined using a competitive ELISA, where a soluble VEGFR2 competes with diluted serum in plates coated with GST-hVEGF (c, e and g). CIGB-247 combinations using VSSP or aluminum phosphate as adjuvants were administered weekly (eight vaccinations) or bi-weekly (four vaccinations) respectively. Horizontal bars represent the mean values of antibody titer or blocking activity, which are shown for each group. p-Values were calculated according to unpaired t-test. Reference dose (A + V): 400 μg of antigen + 200 μg of VSSP; (¼Ag + ½ V): 100 μg of antigen + 100 μg of VSSP; (¼Ag + V): 100 μg of antigen + 200 μg of VSSP; (Ag + Al): 400 μg of antigen + aluminum phosphate Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28747172), licensed under a CC-BY license. Not internally tested by R&D Systems.
Humoral response in pre-clinical models. Specific IgG antibodies were detected by ELISA using GST-hVEGF as coating antigen (a, b, d and f). The ability of animal sera antibodies to block VEGF/VEGFR2 interaction was ...read more
Humoral response in pre-clinical models. Specific IgG antibodies were detected by ELISA using GST-hVEGF as coating antigen (a, b, d and f). The ability of animal sera antibodies to block VEGF/VEGFR2 interaction was ...read more
Humoral response in pre-clinical models. Specific IgG antibodies were detected by ELISA using GST-hVEGF as coating antigen (a, b, d and f). The ability of animal sera antibodies to block VEGF/VEGFR2 interaction was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF357
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF357
Carrier Free
AF357
Carrier Free
AF357-SP
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VEGFR2/KDR/Flk-1 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human VEGFR2/KDR/Flk-1
Ala20-Glu764
Accession # AAC16450
Specificity
Detects human VEGFR2/KDR/Flk-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant mouse VEGFR2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) VEGFR3 and rhVEGFR1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KDR
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human VEGF R2/KDR/Flk-1 Antibody (Catalog # MAB3573)
ELISA Detection: Human VEGF R2/KDR/Flk-1 Biotinylated Antibody (Catalog # BAF357)
Standard: Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD)
Readout System
Publications
Read Publications using
BAF357 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody [Biotin]

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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VEGFR2/KDR/Flk-1
Antibody [Biotin]
BAF357
VEGFR2/KDR/Flk-1 Antibody [Biotin]
Species: Hu
Applications: WB, ELISA(Det)

Publications for VEGFR2/KDR/Flk-1 Antibody (BAF357)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA Development (Detection), Flow Cytometry, TaqMan Protein Assay, Western Blot.


Filter By Application
ELISA Development (Detection)
(2)
Flow Cytometry
(2)
TaqMan Protein Assay
(1)
Western Blot
(1)
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Filter By Species
Human
(4)
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Showing Publications 1 - 7 of 7.
Publications using BAF357 Applications Species
Ahmad F Alghanem, Javier Abello, Joshua M Maurer, Ashutosh Kumar, Chau My Ta, Susheel K Gunasekar et al. The SWELL1-LRRC8 complex regulates endothelial AKT-eNOS signaling and vascular function eLife 2/25/2021 [PMID: 33629656]
J Sánchez Ra, Y Morera Día, M Bequet-Rom, F Hernández-, KH Selman-Hou, A de la Torr, ER Santiesteb, Y Martín Bau, CH Bermúdez B, J de la Torr, JV Gavilondo, M Ayala Avil Characteristics of the specific humoral response in patients with advanced solid tumors after active immunotherapy with a VEGF vaccine, at different antigen doses and using two distinct adjuvants BMC Immunol., 2017-07-26;18(1):39. 2017-07-26 [PMID: 28747172] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Y Morera, J Sánchez, M Bequet-Rom, KH Selman-Hou, A de la Torr, F Hernández-, Y Martín, A Garabito, J Piñero, C Bermúdez, J de la Torr, M Ayala, JV Gavilondo Specific humoral and cellular immune responses in cancer patients undergoing chronic immunization with a VEGF-based therapeutic vaccine Vaccine, 2017-05-20;0(0):. 2017-05-20 [PMID: 28536029] (ELISA Development (Detection)) ELISA Development (Detection)
Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro. Br. J. Cancer, 2012-10-09;107(10):1702-13. 2012-10-09 [PMID: 23047550] (TaqMan Protein Assay, Human) TaqMan Protein Assay Human
Luca Rastelli, Maria Luisa Valentino, Melissa Corso Minderman, Judith Landin, Uriel M Malyankar, Mary K Lescoe et al. A KDR-binding peptide (ST100,059) can block angiogenesis, melanoma tumor growth and metastasis in vitro and in vivo International Journal of Oncology 5/11/2011 [PMID: 21567086]
Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer. Br. J. Cancer, 2006-02-27;94(4):524-31. 2006-02-27 [PMID: 16450002] (Flow Cytometry, Human) Flow Cytometry Human
Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis. Exp. Cell Res., 2004-09-10;299(1):68-78. 2004-09-10 [PMID: 15302574] (Flow Cytometry, Western Blot, Human) Flow Cytometry, Western Blot Human

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Bioinformatics

Gene Symbol KDR
Uniprot