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VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

Images

 
Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) ...read more
VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) overnight at ...read more
VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) at 10 µg/mL overnight ...read more
Recombi­nant Human VEGFR2/KDR/Flk‑1 Fc Chi­mera (Catalog # 357-KD) inhibits Recombinant Human VEGF165 (Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a ...read more
BMP7v exerts antiangiogenic effects and sensitizes chemoresistant CSCs to standard therapy. a Azan-Mallory staining on paraffin-embedded sections of xenografts derived from the injection of CRC sphere cells and treated ...read more
Exogenous VEGF165 suppresses RASSF1A expression in normal epithelial and endothelial cells. Metastatic colon cancer cells (mCRC, (A) T84 and (B) Colo 205) were stimulated with VEGF165 as indicated before and cell ...read more
Exogenous VEGF165 suppresses RASSF1A expression in normal epithelial and endothelial cells. Metastatic colon cancer cells (mCRC, (A) T84 and (B) Colo 205) were stimulated with VEGF165 as indicated before and cell ...read more
Exogenous VEGF165 suppresses RASSF1A expression in normal epithelial and endothelial cells. Metastatic colon cancer cells (mCRC, (A) T84 and (B) Colo 205) were stimulated with VEGF165 as indicated before and cell ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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VEGFR2/KDR/Flk-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human VEGFR2/KDR/Flk-1
Ala20-Glu764
Accession # AAC16450
Specificity
Detects human VEGFR2/KDR/Flk‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse VEGFR2 is observed and less than 10% cross-reactivity with recombinant human (rh) VEGFR1 and rhVEGFR3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KDR
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.05-0.25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 4 Reviews rated 4
using
AF357 in the following applications:

Publications
Read Publications using
AF357 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR2/KDR/Flk-1 Antibody (AF357)(57)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate - Papio anubis (Olive Baboon), Xenograft.

We have publications tested in 7 applications: Functional Assay, ICC, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Functional Assay
(1)
ICC
(7)
IHC
(3)
IHC-P
(2)
Immunoprecipitation
(1)
Neutralization
(10)
Western Blot
(15)
All Applications
Filter By Species
Human
(29)
Mouse
(2)
Rat
(1)
Primate - Papio anubis (Olive Baboon)
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using AF357 Applications Species
Critchley, WR;Smith, GA;Zachary, IC;Harrison, MA;Ponnambalam, S; E2 ubiquitin-conjugating enzymes, UBE2D1 and UBE2D2, regulate VEGFR2 dynamics and endothelial function Journal of cell science 2023-05-09 [PMID: 37158019] (Western Blot, Human) Western Blot Human
Liying Xing, Guanqun Huang, Rongyuan Chen, Lijuan Huang, Juanxi Liu, Xiangrong Ren et al. Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis Journal of Cell Communication and Signaling 2023-03-01 [PMID: 36284029]
Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
Joelle P. Straehla, Cynthia Hajal, Hannah C. Safford, Giovanni S. Offeddu, Natalie Boehnke, Tamara G. Dacoba et al. A predictive microfluidic model of human glioblastoma to assess trafficking of blood–brain barrier-penetrant nanoparticles Proceedings of the National Academy of Sciences 6/7/2022 [PMID: 35648828]
Issahy Cano, Zhengping Hu, Dina B. AbuSamra, Magali Saint-Geniez, Yin Shan Eric Ng, Pablo Argüeso et al. Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor Frontiers in Cell and Developmental Biology 2021-09-20 [PMID: 34616740]
D Ash, V Sudhahar, SW Youn, MN Okur, A Das, JP O'Bryan, M McMenamin, Y Hou, JH Kaplan, T Fukai, M Ushio-Fuka The P-type ATPase transporter ATP7A promotes angiogenesis by limiting autophagic degradation of VEGFR2 Nature Communications, 2021-05-25;12(1):3091. 2021-05-25 [PMID: 34035268] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human
Lanette Kempers, Yuki Wakayama, Ivo van der Bijl, Charita Furumaya, Iris M. De Cuyper, Aldo Jongejan et al. The endosomal RIN2/Rab5C machinery prevents VEGFR2 degradation to control gene expression and tip cell identity during angiogenesis Angiogenesis 2021-08-01 [PMID: 33983539]
MG Dallinga, YI Habani, AWM Schimmel, GM Dallinga-T, CJF van Noorde, I Klaassen, RO Schlingema The Role of Heparan Sulfate and Neuropilin 2 in VEGFA Signaling in Human Endothelial Tip Cells and Non-Tip Cells during Angiogenesis In Vitro Cells, 2021-04-16;10(4):. 2021-04-16 [PMID: 33923753] (ICC, Human) ICC Human
S Bürger, J Meng, A Zwanzig, M Beck, M Pankonin, P Wiedemann, W Eichler, JD Unterlauft Pigment Epithelium-Derived Factor (PEDF) Receptors Are Involved in Survival of Retinal Neurons International Journal of Molecular Sciences, 2020-12-31;22(1):. 2020-12-31 [PMID: 33396450] (Neutralization, Mouse, Rat) Neutralization Mouse, Rat
Z Hu, I Cano, KL Saez-Torre, ME LeBlanc, M Saint-Geni, YS Ng, P Argüeso, PA D'Amore Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity Cells, 2020-06-05;9(6):. 2020-06-05 [PMID: 32517158] (ICC, Human) ICC Human
Show All 57 Publications.

Reviews for VEGFR2/KDR/Flk-1 Antibody (AF357) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using AF357:
Filter by Applications
WB
(1)
IP
(1)
IF
(2)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Human 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19567589
SpeciesHuman
  4
reviewed by:
Verified Customer
IP Human 01/08/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 19857463
SpeciesHuman
  4
reviewed by:
Verified Customer
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23267826
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Human 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23746980
SpeciesHuman

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FAQs for VEGFR2/KDR/Flk-1 Antibody (AF357) (0)

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Recent Reviews

4
5
0
4
4
3
0
2
0
1
0

 
Verified Customer
01/08/2015
Application: WB
Species: Human

 
Verified Customer
01/08/2015
Application: IP
Species: Human

 
Verified Customer
01/08/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol KDR
Uniprot