![Western Blot: UHRF1 Antibody [NBP2-20807] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/UHRF1-Antibody-Western-Blot-NBP2-20807-img0009.jpg "Western Blot: UHRF1 Antibody [NBP2-20807] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human UHRF1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UHRF1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Chip Assay-Assay depenedent |
| Theoretical MW | 90 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Intracellular Staining by Flow Cytometry IL-17B Antibody [Unconjugated]](https://images.novusbio.com/images2/IL-17B_AF1709_Flow_Cytometry_8161.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for UHRF1 Antibody (NBP2-20807)Find related products by research area.
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Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA... Read full blog post. |
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![Immunohistochemistry-Paraffin: UHRF1 Antibody [NBP2-20807] - HBL435 xenograft. UHRF1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Immunohistochemistry-Paraffin-NBP2-20807-img0007.jpg "Immunohistochemistry-Paraffin: UHRF1 Antibody [NBP2-20807] - HBL435 xenograft. UHRF1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Chromatin Immunoprecipitation: UHRF1 Antibody [NBP2-20807] - Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ug of either control rabbit IgG or anti-UHRF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Chromatin-Immunoprecipitation-NBP2-20807-img0005.jpg "Chromatin Immunoprecipitation: UHRF1 Antibody [NBP2-20807] - Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ug of either control rabbit IgG or anti-UHRF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.")
![Immunocytochemistry/Immunofluorescence: UHRF1 Antibody [NBP2-20807] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UHRF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20807-img0002.jpg "Immunocytochemistry/Immunofluorescence: UHRF1 Antibody [NBP2-20807] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UHRF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Immunoprecipitation: UHRF1 Antibody [NBP2-20807] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of UHRF1 protein by 2 ug of UHRF1 antibody 5% SDS-PAGE The immunoprecipitated UHRF1 protein was detected by UHRF1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Immunoprecipitation-NBP2-20807-img0004.jpg "Immunoprecipitation: UHRF1 Antibody [NBP2-20807] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of UHRF1 protein by 2 ug of UHRF1 antibody 5% SDS-PAGE The immunoprecipitated UHRF1 protein was detected by UHRF1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.")
![Western Blot: UHRF1 Antibody [NBP2-20807] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody (NBP2-20807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20807_rabbit-polyclonal-uhrf1-antibody-91020242091425.jpg "Western Blot: UHRF1 Antibody [NBP2-20807] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody (NBP2-20807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/ Immunofluorescence: UHRF1 Antibody [NBP2-20807] - UHRF1 antibody detects UHRF1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: UHRF1 stained by UHRF1 antibody (NBP2-20807) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20807_rabbit-polyclonal-uhrf1-antibody-91020242041228.jpg "Immunocytochemistry/ Immunofluorescence: UHRF1 Antibody [NBP2-20807] - UHRF1 antibody detects UHRF1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: UHRF1 stained by UHRF1 antibody (NBP2-20807) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: UHRF1 Antibody [NBP2-20807] - UHRF1 antibody detects UHRF1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.UHRF1 stained by UHRF1 antibody (NBP2-20807) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20807_rabbit-polyclonal-uhrf1-antibody-91020242021921.jpg "Immunohistochemistry-Paraffin: UHRF1 Antibody [NBP2-20807] - UHRF1 antibody detects UHRF1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.UHRF1 stained by UHRF1 antibody (NBP2-20807) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: UHRF1 Antibody [NBP2-20807] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20807_rabbit-polyclonal-uhrf1-antibody-1610202419414584.jpg "Western Blot: UHRF1 Antibody [NBP2-20807] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Western Blot: UHRF1 Antibody [NBP2-20807] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20807_rabbit-polyclonal-uhrf1-antibody-1610202419405775.jpg "Western Blot: UHRF1 Antibody [NBP2-20807] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")