![Western Blot: UHRF1 Antibody [NBP2-20806] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Western-Blot-NBP2-20806-img0004.jpg "Western Blot: UHRF1 Antibody [NBP2-20806] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human UHRF1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UHRF1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 91 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Intracellular Staining by Flow Cytometry IL-17B Antibody [Unconjugated]](https://images.novusbio.com/images2/IL-17B_AF1709_Flow_Cytometry_8161.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for UHRF1 Antibody (NBP2-20806)Find related products by research area.
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Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: UHRF1 Antibody [NBP2-20806] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UHRF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20806-img0003.jpg "Immunocytochemistry/Immunofluorescence: UHRF1 Antibody [NBP2-20806] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UHRF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: UHRF1 Antibody [NBP2-20806] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Immunohistochemistry-Paraffin-NBP2-20806-img0002.jpg "Immunohistochemistry-Paraffin: UHRF1 Antibody [NBP2-20806] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:250 dilution.")
![Western Blot: UHRF1 Antibody [NBP2-20806] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Western-Blot-NBP2-20806-img0001.jpg "Western Blot: UHRF1 Antibody [NBP2-20806] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:2000.")
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![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241251265.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")