![Western Blot: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25732_rabbit-uhrf1-mab-hl2287-azide-and-bsa-free-212024940382.jpg "Western Blot: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | HL2287 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human UHRF1. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | UHRF1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Intracellular Staining by Flow Cytometry IL-17B Antibody [Unconjugated]](https://images.novusbio.com/images2/IL-17B_AF1709_Flow_Cytometry_8161.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for UHRF1 Antibody (NBP3-25732)Find related products by research area.
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Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA... Read full blog post. |
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| Gene Symbol | UHRF1 |
![Immunohistochemistry-Paraffin: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. UHRF1 stained by UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25732_rabbit-uhrf1-mab-hl2287-azide-and-bsa-free-212024937499.jpg "Immunohistochemistry-Paraffin: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. UHRF1 stained by UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Knockdown Validated: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25732_rabbit-uhrf1-mab-hl2287-azide-and-bsa-free-212024940817.jpg "Knockdown Validated: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. UHRF1 stained by UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25732_rabbit-uhrf1-mab-hl2287-azide-and-bsa-free-2120249355544.jpg "Immunohistochemistry-Paraffin: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. UHRF1 stained by UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25732_rabbit-uhrf1-mab-hl2287-azide-and-bsa-free-2120249362713.jpg "Western Blot: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")