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UCP2 Antibody - BSA Free

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Immunocytochemistry/Immunofluorescence: UCP2 Antibody [NBP2-69001] - Staining of human cell line RT4 shows localization to mitochondria.
Immunocytochemistry/ Immunofluorescence: UCP2 Antibody [NBP2-69001] - Staining of human cell line RT4 shows localization to mitochondria.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-69001-25ul
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UCP2 Antibody - BSA Free Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ESQGPVRATASAQYRGVMGT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UCP2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
Recommended conditions: Fixation/Permeabilization: PFA/Triton X-100
Control Peptide
UCP2 Recombinant Protein Antigen (NBP2-69001PEP)

Reactivity Notes

Mouse 80%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for UCP2 Antibody - BSA Free

  • mitochondrial uncoupling protein 2
  • SLC25A8
  • SLC25A8BMIQ4
  • Solute carrier family 25 member 8
  • UCP 2
  • UCP2
  • UCPH
  • uncoupling protein 2 (mitochondrial, proton carrier)

Background

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for UCP2 Antibody (NBP2-69001). (Showing 1 - 1 of 1 FAQ).

  1. Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
    • Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

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Bioinformatics

Gene Symbol UCP2