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BAAT Antibody - BSA Free

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Western Blot: BAAT Antibody [NBP2-92503] - Analysis of extracts of various cell lines, using BAAT at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more
Immunocytochemistry/ Immunofluorescence: BAAT Antibody [NBP2-92503] - Analysis of Hep G2 cells using BAAT Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: BAAT Antibody [NBP2-92503] - Human liver using BAAT Rabbit pAb at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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BAAT Antibody - BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 309-418 of human BAAT (NP_001692.1). YLFPIEEAQGQFLFIVGEGDKTINSKAHAEQAIGQLKRHGKNNWTLLSYPGAGHLIEPPYSPLCCASTTHDLRLHWGGEVIPHAAAQEHAWKEIQRFLRKHLIPDVTSQL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAAT
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Western Blot 1:500-1:2000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for BAAT Antibody - BSA Free

  • BACAT
  • BATbile acid-CoA:amino acid N-acyltransferase
  • bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase)
  • bile acid Coenzyme A: amino acid N-acyltransferase (glycineN-choloyltransferase)
  • EC 2.3.1.65
  • EC 3.1.2.2
  • FLJ20300
  • Glycine N-choloyltransferase
  • Long-chain fatty-acyl-CoA hydrolase
  • MGC104432

Background

BAAT is encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BAAT Antibody (NBP2-92503) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BAAT