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UCP1 Antibody (536435) [Unconjugated]

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Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal ...read more
Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by ...read more
UCP1 was detected in immersion fixed human mesenchymal stem cells undifferentiated (lower panel) or differentiated into adipocytes (upper panel) using Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) ...read more
3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more
Simple Western lane view shows lysates of mouse adipose tissue and mouse brown adipose tissue, loaded at 0.5 mg/mL. A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse ...read more
IRX3/Irx3 expression is induced in browning adipose tissues.(A) Relative mRNA levels of Ucp1 and Irx3 in IWAT from mice under cold stress (4 °C) for one week (n = 6) and at 25 °C (n = 8). (B–C) Relative mRNA ...read more
IRX3/Irx3 expression is induced in browning adipose tissues.(A) Relative mRNA levels of Ucp1 and Irx3 in IWAT from mice under cold stress (4 °C) for one week (n = 6) and at 25 °C (n = 8). (B–C) Relative mRNA ...read more
BMP7 upregulated browning markers in both SC and DN derived differentiated adipocytes. SC and DN preadipocytes were differentiated and treated as in Figure 1. (A) Browning content as estimated by BATLAS Webtool (n = 9); ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF
Clone
536435
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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UCP1 Antibody (536435) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human UCP1
Met1-Thr307
Accession # P25874
Specificity
Detects human and mouse UCP1 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UCP1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 2.5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB6158 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UCP1 Antibody (536435) [Unconjugated]

  • SLC25A7
  • SLC25A7mitochondrial brown fat uncoupling protein 1
  • Solute carrier family 25 member 7
  • Thermogenin
  • UCP 1
  • UCP
  • UCP1
  • uncoupling protein 1 (mitochondrial, proton carrier)

Background

Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB6158
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Publications for UCP1 Antibody (MAB6158)(53)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Immunohistochemistry, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(5)
IHC
(2)
IHC-Fr
(1)
Immunohistochemistry
(1)
Western Blot
(24)
All Applications
Filter By Species
Human
(13)
Mouse
(19)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using MAB6158 Applications Species
Song, Y;Zhu, M;Islam, MA;Gu, W;Alim, K;Cheng, CS;Chen, J;Xu, Y;Xu, H; Glutathione peroxidase 3 is essential for countering senescence in adipose remodelling by maintaining mitochondrial homeostasis Redox biology 2024-09-19 [PMID: 39312866] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Chau, PK;Ryan, E;Dalen, KT;Haugen, F; Timing of acute cold exposure determines UCP1 and FGF21 expression - Possible interactions between the thermal environment, thermoregulatory responses, and peripheral clocks Journal of thermal biology 2024-08-10 [PMID: 39142264] (Western Blot, Mouse) Western Blot Mouse
Takeda, Y;Yoshikawa, T;Dai, P; Angiotensin II participates in mitochondrial thermogenic functions via the activation of glycolysis in chemically induced human brown adipocytes Scientific reports 2024-05-11 [PMID: 38734719] (Western Blot, Human) Western Blot Human
Herrnhold, M;Hamp, I;Plettenburg, O;Jastroch, M;Keuper, M; Adverse bioenergetic effects of N-acyl amino acids in human adipocytes overshadow beneficial mitochondrial uncoupling Redox biology 2023-09-02 [PMID: 37683300] (ICC, Human) ICC Human
Vámos A, Arianti R, Vinnai B et al. Human abdominal subcutaneous-derived active beige adipocytes carrying FTO rs1421085 obesity-risk alleles exert lower thermogenic capacity Frontiers in Cell and Developmental Biology 2023-06-14 [PMID: 37416800]
Ann A. Coulter, Frank L. Greenway, Dachuan Zhang, Sujoy Ghosh, Cathryn R. Coulter, Sarah L. James et al. Naringenin and beta -carotene convert human white adipocytes to a beige phenotype and elevate hormone- stimulated lipolysis Front Endocrinol (Lausanne) 2023-04-17 [PMID: 37143734]
P Janovska, P Zouhar, K Bardova, J Otahal, M Vrbacky, T Mracek, K Adamcova, L Lenkova, J Funda, T Cajka, Z Drahota, S Stanic, AC Rustan, O Horakova, J Houstek, M Rossmeisl, J Kopecky Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle Molecular Metabolism, 2023-01-30;69(0):101683. 2023-01-30 [PMID: 36720306] (Western Blot, Mouse) Western Blot Mouse
KM Choi, CY Ko, SM An, SH Cho, DJ Rowland, JH Kim, A Fasoli, AJ Chaudhari, DM Bers, JC Yoon Regulation of beige adipocyte thermogenesis by the cold-repressed ER protein NNAT Molecular Metabolism, 2023-01-25;0(0):101679. 2023-01-25 [PMID: 36708951] (Western Blot, Mouse) Western Blot Mouse
H Takahashi, G Yang, T Yoneshiro, Y Abe, R Ito, C Yang, J Nakazono, M Okamoto-Ka, A Uchida, M Arai, H Jin, H Choi, M Tumenjarga, S Xie, J Zhang, H Sagae, Y Zhao, R Yamaguchi, Y Nomura, Y Shimizu, K Yamada, S Yasuda, H Kimura, T Tanaka, Y Wada, T Kodama, H Aburatani, MS Zhu, T Inagaki, TF Osborne, T Kawamura, Y Ishihama, Y Matsumura, J Sakai MYPT1-PP1beta phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis Nature Communications, 2022-09-29;13(1):5715. 2022-09-29 [PMID: 36175407] (Western Blot, Mouse) Western Blot Mouse
Xin Yu, Manman Shi, Qi Wu, Wen Wei, Shengrong Sun, Shan Zhu Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics Frontiers in Cell and Developmental Biology 2022-07-07 [PMID: 35874806]
Show All 53 Publications.

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Bioinformatics

Gene Symbol UCP1
Uniprot