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ROS Antibody (OTI1F3)

Images

 
Western Blot: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS.
Immunohistochemistry-Paraffin: ROS Antibody (OTI1F3) [NBP2-45626] - Staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (Left). The right is HELA cells as negative control (1:2000).
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Carcinoma Human lung tissue.
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Carcinoma Human lung tissue.
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Human carcinoma lung tissue.
Western Blot: ROS Antibody (OTI1F3) [NBP2-45626] -Analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody (1:500).
Immunohistochemistry-Paraffin: ROS Antibody (OTI1F3) [NBP2-45626] -HCC78 (left) and HeLa (right) xenograft using NBP2-45626 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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ROS Antibody (OTI1F3) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 2126-2347 of human ROS1(NP_002935) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ROS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:5000
  • Immunohistochemistry-Paraffin 1:5000
  • Western Blot 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ROS Antibody (OTI1F3)

  • proto-oncogene tyrosine-protein kinase ROS
  • c-ros oncogene 1 , receptor tyrosine kinase
  • c-Ros-1
  • MCF3
  • ROS
  • ROS1

Background

This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ROS Antibody (NBP2-45626) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-45626

Research Areas for ROS Antibody (NBP2-45626)

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Blogs on ROS.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Methamphetamine with HIV induces mitochondrial dysfunction and neuronal injury through oxidative stress
By Jamshed Arslan, Pharm. D., PhD. December 1 is the World AIDS Day. Despite the combination antiretroviral therapy, 10-25% of Human Immunodeficiency Virus (HIV)-positive individuals report neurocognitive impairm...  Read full blog post.

NOX2 oxidase is a potential target for broad-spectrum antiviral therapies
By Beth Melson, MSViral infection induces the production of reactive oxygen species (ROS) by inflammatory cells, the majority of which is produced by the NOX2 oxidase. While NOX2 oxidase contributes to the clearan...  Read full blog post.

Crosstalk Between Oxidative Stress and Autophagy
By Christina Towers, PhD. Role of Reactive Species in Cellular FunctionOxidative stress is a byproduct of an imbalance between oxidants and antioxidants present in the cell, resulting in dysfunctional redox si...  Read full blog post.

One MAP to Navigate the Oxidative Stress, Tumorigenesis and Apoptosis Pathways?
Reactive oxygen species, ROS, are beneficially involved in many signaling pathways that control development and maintain cellular homeostasis. In physiological conditions, a tightly regulated redox balance protects cells from injurious ROS activity, a...  Read full blog post.

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Bioinformatics

Gene Symbol ROS1