![ELISA: Human Twist-1 ELISA Kit (Colorimetric) [NBP3-06809] - Samples were spiked with high concentrations of Human Twist-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06809_human-twist-1-elisa-kit-colorimetric-132202416215315.jpg "ELISA: Human Twist-1 ELISA Kit (Colorimetric) [NBP3-06809] - Samples were spiked with high concentrations of Human Twist-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma, cell supernatant, and other biological fluids |
| Standard Curve Range | 62.5 - 4000 pg/mL |
| Sensitivity | 37.5 pg/mL |
| Specificity | This kit recognizes Human Twist-1 in samples. No significant cross-reactivity or interference between Human Twist-1 and analogues was observed. |
| Standard Curve Range | 62.5 - 4000 pg/mL |
| Sensitivity | 37.5 pg/mL |
| Assay Type | Sandwich-ELISA |
| Inter-Assay | 5.46 |
| Intra-Assay | 5.31 |
| Spike Recovery | 88 - 107 % |
| Sample Volume | 100 ul |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | TWIST1 |
| Dilutions |
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| Publications |
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| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
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| Gene Symbol | TWIST1 |
![ELISA: Human Twist-1 ELISA Kit (Colorimetric) [NBP3-06809] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06809_human-twist-1-elisa-kit-colorimetric-4320249124892.jpg "ELISA: Human Twist-1 ELISA Kit (Colorimetric) [NBP3-06809] - Standard Curve Reference")
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![Western Blot Snail Antibody [Unconjugated]](https://images.novusbio.com/images2/Snail_AF3639_Western_Blot_5320.jpg)
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![Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.](https://images.novusbio.com/images/Twist-1-Antibody-10E4E6-Flow-Cytometry-NBP2-37364-img0012.jpg "Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.")