Can snail antibody AF3639 be used for electrophoresis motility shift assays (EMSA) instead of ChIP?

For IP/Co-IP and ChIP the antibodies need to provide sufficient binding affinity for the antibodies to stay bound to the protein targets during the precipitation steps, whereas in EMSA the antibodies will only need to bind to the protein targets during the migration through the weak electrophoretic forces. Almost all, if not all, antibodies that bind to their protein targets with reasonable affinity and do not fall off from their protein targets when bound to the targeted, labeled DNA/RNA molecules during PAGE will be rendered in the upper shift in EMSA, provided the binding doesn't block the interaction between the protein targets and the nucleic acids .

There are many kinds of antibodies you supply against SNAIL. Could you give me your recommendation? Which is the best for the IHC-P experiments the species of my samples to be tested is human.

Our SNAIL antibody with catalog # NBP1-19529 has been successfully validated for IHC-P in paraffin-embedded human lung carcinoma tissue and I would highly recommend you to use the same for your samples too. The working dilutions of this antibody for IHC-P ranges from 1:50 - 1:200 and beside IHC, you can use this antibody for Western Blot and Immunofluorescence also.

Snail Antibody [Unconjugated] (AF3639): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, ChIP, Flow, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free AF3639-SP

Carrier Free IC3639G-025

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	IC3639G-025

Immunogen	E. coli-derived recombinant human Snail Pro2-Arg264 Accession # O95863
Specificity	Detects human Snail in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	SNAI1
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Snail Antibody [Unconjugated]

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Publications for Snail Antibody (AF3639)(35)

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Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF3639	Applications	Species
Despin-Guitard, E;Rosa, VS;Plunder, S;Mathiah, N;Van Schoor, K;Nehme, E;Merino-Aceituno, S;Egea, J;Shahbazi, MN;Theveneau, E;Migeotte, I; Non-apical mitoses contribute to cell delamination during mouse gastrulation Nature communications 2024-08-28 [PMID: 39198421] (Immunohistochemistry, Mouse)	Immunohistochemistry	Mouse
Rokavec, M;Jaeckel, S;Hermeking, H; Nidogen-1/NID1 Function and Regulation during Progression and Metastasis of Colorectal Cancer Cancers 2023-11-07 [PMID: 38001576] (Chromatin Immunoprecipitation (ChIP), Human)	Chromatin Immunoprecipitation (ChIP)	Human
JD Twomey, B Zhang Exploring the Role of Hypoxia-Inducible Carbonic Anhydrase IX (CAIX) in Circulating Tumor Cells (CTCs) of Breast Cancer Biomedicines, 2023-03-17;11(3):. 2023-03-17 [PMID: 36979915] (Western Blot, Human)	Western Blot	Human
Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
M Simunovic, ED Siggia, AH Brivanlou Inï¿½vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells Cell Stem Cell, 2022-06-02;29(6):962-972.e4. 2022-06-02 [PMID: 35659878] (IHC, Human)	IHC	Human
Menggui Huang, Fan Yang, Duo Zhang, Maohuan Lin, Hao Duan, Rakan El-Mayta et al. Endothelial plasticity drives aberrant vascularization and impedes cardiac repair after myocardial infarction Nature Cardiovascular Research 5/16/2022 [PMID: 35571674]
Sophie M Morgani, Jie Su, Jennifer Nichols, Joan Massagué, Anna-Katerina Hadjantonakis The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos eLife 4/30/2021 [PMID: 33929320]
Liqun Li, Qiang Liu, Tongyao Shang, Wei Song, Dongmei Xu, Thaddeus D. Allen et al. Aberrant Activation of Notch1 Signaling in Glomerular Endothelium Induces Albuminuria Circulation Research 3/5/2021 [PMID: 33435713]
Tian X, Yu H, Li D et al. The miR-5694/AF9/Snail Axis Provides Metastatic Advantages and a Therapeutic Target in Basal-like Breast Cancer Molecular Therapy 2021-03-01 [PMID: 33221433]
Tian X, Yu H, Li D et al. The miR-5694/AF9/Snail Axis Provides Metastatic Advantages and a Therapeutic Target in Basal-like Breast Cancer Molecular Therapy 3/1/2021 [PMID: 33221433]
Battistelli C, Garbo S, Riccioni V et al. Design and Functional Validation of a Mutant Variant of the LncRNA HOTAIR to Counteract Snail Function in Epithelial-to-Mesenchymal Transition Cancer Research 2021-01-01 [PMID: 33158813]
Battistelli C, Garbo S, Riccioni V et al. Design and Functional Validation of a Mutant Variant of the LncRNA HOTAIR to Counteract Snail Function in Epithelial-to-Mesenchymal Transition Cancer Research 1/1/2021 [PMID: 33158813]
Emma D. Zanfi, Sebastian Fantini, Roberta Lotti, Matteo Bertesi, Alessandra Marconi, Alexis Grande et al. Wnt/CTNNB1 Signal Transduction Pathway Inhibits the Expression of ZFP36 in Squamous Cell Carcinoma, by Inducing Transcriptional Repressors SNAI1, SLUG and TWIST International Journal of Molecular Sciences 8/8/2020 [PMID: 32784485]
Klaudia Skrzypek, Marta Kot, Paweł Konieczny, Artur Nieszporek, Anna Kusienicka, Małgorzata Lasota et al. SNAIL Promotes Metastatic Behavior of Rhabdomyosarcoma by Increasing EZRIN and AKT Expression and Regulating MicroRNA Networks Cancers (Basel) 7/11/2020 [PMID: 32664538]
NC Boles, M Fernandes, T Swigut, R Srinivasan, L Schiff, A Rada-Igles, Q Wang, JS Saini, T Kiehl, JH Stern, J Wysocka, TA Blenkinsop, S Temple Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide Stem Cell Reports, 2020-04-02;14(4):631-647. 2020-04-02 [PMID: 32243845] (ICC, Human)	ICC	Human
Sofiane Hamidi, Yukiko Nakaya, Hiroki Nagai, Cantas Alev, Takeya Kasukawa, Sapna Chhabra et al. Mesenchymal-epithelial transition regulates initiation of pluripotency exit before gastrulation Development 2/1/2020 [PMID: 32014865]
R Zhang, J Liu, W Zhang, L Hua, LT Qian, SB Zhou EphA5 knockdown enhances the invasion and migration ability of esophageal squamous cell carcinoma via epithelial-mesenchymal transition through activating Wnt/beta-catenin pathway Cancer Cell Int., 2020-01-13;20(0):20. 2020-01-13 [PMID: 31956298] (IF, Human)	IF	Human
K Fukuura, Y Inoue, C Miyajima, S Watanabe, M Tokugawa, D Morishita, N Ohoka, M Komada, H Hayashi The ubiquitin-specific protease USP17 prevents cellular senescence by stabilizing the methyltransferase SET8 and transcriptionally repressing p21 J. Biol. Chem., 2019-09-18;0(0):. 2019-09-18 [PMID: 31533987] (Western Blot, Human)	Western Blot	Human
G Rademaker, B Costanza, S Anania, F Agirman, N Maloujahmo, E Di Valenti, JJ Goval, A Bellahcène, V Castronovo, O Peulen Myoferlin Contributes to the Metastatic Phenotype of Pancreatic Cancer Cells by Enhancing Their Migratory Capacity through the Control of Oxidative Phosphorylation Cancers (Basel), 2019-06-19;11(6):. 2019-06-19 [PMID: 31248212] (Western Blot, Human)	Western Blot	Human
JM Oh, J Lee, WT Im, S Chun Ginsenoside Rk1 Induces Apoptosis in Neuroblastoma Cells Through Loss of Mitochondrial Membrane Potential and Activation of Caspases Int J Mol Sci, 2019-03-11;20(5):. 2019-03-11 [PMID: 30862004] (Western Blot, Human)	Western Blot	Human
M Kurihara-S, T Sasahira, H Shimomura, C Nakashima, T Kirita The Oncogenic Activity of miR-29b-1-5p Induces the Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma J Clin Med, 2019-02-24;8(2):. 2019-02-24 [PMID: 30813466] (Western Blot, Human)	Western Blot	Human
T Liu, W Ma, H Xu, M Huang, D Zhang, Z He, L Zhang, S Brem, DM O'Rourke, Y Gong, Y Mou, Z Zhang, Y Fan PDGF-mediated mesenchymal transformation renders endothelial resistance to anti-VEGF treatment in glioblastoma Nat Commun, 2018-08-27;9(1):3439. 8/27/2018 [PMID: 30150753]
Sophie M Morgani, Jakob J Metzger, Jennifer Nichols, Eric D Siggia, Anna-Katerina Hadjantonakis Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning eLife 2/7/2018 [PMID: 29412136]
Shyam Kumar Gudey, Reshma Sundar, Carl-Henrik Heldin, Anders Bergh, Marene Landström Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGF? stimulation in cancer. Oncotarget, 2017-08-09;0(0):1949-2553. 2017-08-09 [PMID: 29228645] (Immunoprecipitation, Human, Mouse)	Immunoprecipitation	Human, Mouse
Gonnissen A, Isebaert S, Perneel C et al. Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer BMC Cancer 9/6/2017 [PMID: 28877722]
Ying Chen, Dan-Dan Wang, Ye-Ping Wu, Dan Su, Tian-Yi Zhou, Ren-Hua Gai, Ying-Ying Fu, Lin Zheng, Qiao-Jun He, Hong Zhu, Bo Yang MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells. British Journal of Cancer, 2017-08-17;0(0):1532-1827. 2017-08-17 [PMID: 28817834] (Western Blot, Human)	Western Blot	Human
M Rokavec, M Kaller, D Horst, H Hermeking Pan-cancer EMT-signature identifies RBM47 down-regulation during colorectal cancer progression Sci Rep, 2017-07-05;7(1):4687. 2017-07-05 [PMID: 28680090] (ChIP, Human)	ChIP	Human
Anitha K. Shenoy, Yue Jin, Huacheng Luo, Ming Tang, Christine Pampo, Rong Shao et al. Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells Journal of Clinical Investigation 10/10/2016 [PMID: 27721239]
Clare Verrill, Lucia Cerundolo, Chad Mckee, Michael White, Christiana Kartsonaki, Eve Fryer et al. Altered expression of epithelial-to-mesenchymal transition proteins in extraprostatic prostate cancer Oncotarget 12/19/2015 [PMID: 26701730]
Sabine Hünten, Heiko Hermeking p53 directly activates cystatin D/CST5 to mediate mesenchymal-epithelial transition: a possible link to tumor suppression by vitamin D3 Oncotarget 6/30/2015 [PMID: 26158294]
Kwon CH, Park HJ, Choi JH et al. Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer. Oncotarget 8/21/2015 [PMID: 26015410]
Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman M, Kopetz S, Ellis L, Meng Q, Li S Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression. Clin Cancer Res, 2014-12-16;21(4):899-906. 2014-12-16 [PMID: 25516888] (ICC, Human)	ICC	Human
Kwon CH, Park HJ, Lee JR et al. Serpin peptidase inhibitor clade A member 1 is a biomarker of poor prognosis in gastric cancer. Br J Cancer 11/11/2014 [PMID: 25211665]
Sarah Javaid, Jianmin Zhang, Endre Anderssen, Josh C. Black, Ben S. Wittner, Ken Tajima et al. Dynamic Chromatin Modification Sustains Epithelial-Mesenchymal Transition following Inducible Expression of Snail-1 Cell Reports 12/26/2013 [PMID: 24360956]
Schwock J, Bradley G, Ho JC SNAI1 expression and the mesenchymal phenotype: an immunohistochemical study performed on 46 cases of oral squamous cell carcinoma. BMC Clin Pathol, 2010-02-05;10(0):1. 2010-02-05 [PMID: 20181105] (IHC-P, Western Blot, Human)	IHC-P, Western Blot	Human
Show All 35 Publications. Collapse Publications.

Gene Symbol	SNAI1
Uniprot	O95863()