Genetic Strategies: Western Blot: Mkl1 Antibody [NBP1-88498] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: Mkl1 Antibody [NBP1-88498] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human testis shows positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human liver shows very weak positivity in hepatocytes.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lymph node shows moderate positivity in lymphoid cells.
This antibody was developed against Recombinant Protein corresponding to amino acids: EQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAVAPGPPSVVVKQEALQPEPEPVPAPQLLLGPQGPSLIKGVAPPTLITDSTGTHLVLTVTNKNADSPG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MRTFA
Purity
Immunogen affinity purified
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Mouse reactivity reported in the scientific literature (PMID: 26165921)
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Mkl1 Antibody
AMKL
KIAA1438
MALSAP and coiled-coil domain
megakaryoblastic leukemia (translocation) 1
Megakaryoblastic leukemia 1 protein
Megakaryocytic acute leukemia protein
MKL/myocardin-like protein 1
Myocardin-related transcription factor A
RNA-binding motif protein 15/megakaryoblastic leukemia-1 fusion protein
Background
MKL1 (megakaryoblastic leukemia 1 protein) was identified as part of a t(1;22) translocation with RBM15 associated with acute megakaryoblastic leukemia. It is a transcriptional coactivator of serum response factor (SRF) that promotes muscle differentiation and also plays a role in promoting maturation of megakaryocytes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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