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MAL Antibody (B5-G3)

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Western Blot: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of MAL on mouse kidney (1) and mouse lymphatic vessels (2) tissue lysate using anti-MAL antibody at 1/1,000 dilution.
Western Blot: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of MAL on mouse kidney (1) and mouse lymphatic vessels (2) tissue lysate using anti-MAL antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of paraffin-embedded rat kidney tissue using anti-MAL antibody. Counter stained with hematoxylin.
Flow Cytometry: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of Jurkat cells with MAL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of paraffin-embedded mouse brain tissue using anti-MAL antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of paraffin-embedded mouse kidney tissue using anti-MAL antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of paraffin-embedded rat brain tissue using anti-MAL antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC
Clone
B5-G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2 mg/ml

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MAL Antibody (B5-G3) Summary

Immunogen
Synthetic peptide within mouse MAL aa 97-146 / 153. (SwissProt: P21145 Human; SwissProt: O09198 Mouse; SwissProt: Q64349 Rat)
Localization
Membrane.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAL
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH7.4), 0.2% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAL Antibody (B5-G3)

  • mal, T-cell differentiation protein
  • myelin and lymphocyte protein
  • T-cell differentiation protein MAL
  • T-lymphocyte maturation-associated protein

Background

The protein encoded by the MAL gene is a highly hydrophobic integral membrane protein belonging to the MAL family of proteolipids. The protein has been localized to the endoplasmic reticulum of T-cells and is a candidate linker protein in T-cell signal transduction. In addition, this proteolipid is localized in compact myelin of cells in the nervous system and has been implicated in myelin biogenesis and/or function. The protein plays a role in the formation, stabilization and maintenance of glycosphingolipid-enriched membrane microdomains. Alternative splicing produces four transcript variants which vary from each other by the presence or absence of alternatively spliced exons 2 and 3. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAL