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Twist-2 Antibody (3C8)

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Western Blot: Twist-2 Antibody (3C8) [H00117581-M01] - Analysis of TWIST2 expression in transfected 293T cell line by TWIST2 monoclonal antibody (M01), clone 3C8.Lane 1: TWIST2 transfected lysate(18.1 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01] - Analysis of monoclonal antibody to TWIST2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.
Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01] - Immunoperoxidase of monoclonal antibody to TWIST2 ( Cat # H00117581-M01 ) on formalin-fixed paraffin-embedded human liver. [antibody concentration ...read more
ELISA: Twist-2 Antibody (3C8) [H00117581-M01] - Detection limit for recombinant GST tagged TWIST2 is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, ChIP
Clone
3C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Twist-2 Antibody (3C8) Summary

Immunogen
TWIST2 (AAH33168, 1 a.a. ~ 160 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEGSSSPVSPVDSLGTSEEELERQPKRFGRKRRYSKKSSEDGSPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTSCSYVAHERLSYAFSVWRMEGSWSMSASH
Specificity
TWIST2 - twist homolog 2 (Drosophila)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TWIST2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using
H00117581-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Twist-2 Antibody (3C8)

  • bHLHa39
  • bHLHa39twist-related bHLH protein Dermo1
  • Class A basic helix-loop-helix protein 39
  • Dermis-expressed protein 1
  • DERMO1
  • Dermo-1
  • DERMO1MGC117334
  • twist homolog 2 (Drosophila)
  • Twist2
  • Twist-2
  • twist-related protein 2

Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Twist-2 Antibody (H00117581-M01)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: CyTOF-ready, IF/IHC.


Filter By Application
CyTOF-ready
(1)
IF/IHC
(2)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using H00117581-M01 Applications Species
Radnaa E, Richardson L, Goldman B Et al. Stress signaler p38 mitogen-activated kinase activation: a cause for concern? Clin Sci (Lond) 2022-10-17 [PMID: 36250628] (CyTOF-ready, Human)

Details:
Citation using the Azide and BSA Free version of this antibody.
CyTOF-ready Human
Jin K, Minji P, Jungyoon O et al. Twist2-driven chromatin remodeling governs the postnatal maturation of dermal fibroblasts. Cell Rep. 2022-05-17 [PMID: 35584664]
Sunsook H, Changjin L, Kyungsoo P et al. Twist2 Promotes CD8 + T-cell Differentiation by Repressing ThPOK Expression. Cell Death Differ. 2020-05-18 [PMID: 32424141]
Zhang J, Lin X, Wu L et al. Aurora B induces epithelial-mesenchymal transition by stabilizing Snail1 to promote basal-like breast cancer metastasis. Oncogene. 2020-01-29 [PMID: 31996785]
Singh M, Venugopal C, Tokar T et al. RNAi screen identifies essential regulators of human brain metastasis-initiating cells. Acta Neuropathol 2017-08-01 [PMID: 28766011]
Oh S, Oh J, Lee C et al. Expression of Twist2 is controlled by T-cell receptor signaling and determines the survival and death of thymocytes. Cell Death Differ 2016-07-08 [PMID: 27391798]
Rodrigues IS, Lavorato-Rocha AM, de M Maia B et al. Epithelial-mesenchymal transition-like events in vulvar cancer and its relation with HPV. Br J Cancer. 2013-06-18 [PMID: 23778524]
Yu H, Jin GZ, Liu K et al. Twist2 is a valuable prognostic biomarker for colorectal cancer. World J Gastroenterol. 2013-04-21 [PMID: 23613636] (IF/IHC, Human) IF/IHC Human
Mao Y, Zhang N, Xu J et al. Significance of heterogeneous twist2 expression in human breast cancers. PLoS One. 2012-10-25 [PMID: 23133563]
Plasilova M, Chattopadhyay C, Ghosh A et al. Discordant Gene Expression Signatures and Related Phenotypic Differences in Lamin A-and A/C-Related Hutchinson-Gilford Progeria Syndrome (HGPS). PLoS One. 2011-06-27 [PMID: 21738662]
Show All 14 Publications.

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Product General Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TWIST2
Entrez
OMIM
Uniprot