TTC11 Antibody

TTC11 is a 17KDa integral membrane protein, and is a novel member of the mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ELISA, ICC/IF, IF/IHC, IHC-P, Immunohistochemistry, WB, Western Blot.

Showing Publications 1 - 10 of 32. Show All 32 Publications. Collapse Publications.

Publications using NB100-56646	Applications	Species
Differential Mitochondrial Adaptation in Primary Vascular Smooth Muscle Cells from a Diabetic Rat Model. Keller AC, Knaub LA, McClatchey PM Oxid Med Cell Longev [PMID: 27034743] (WB, Rat)	WB	Rat
Placido AI, Pereira CM, Correira SC et al. Phosphatase 2A Inhibition Affects Endoplasmic Reticulum and Mitochondria Homeostasis Via Cytoskeletal Alterations in Brain Endothelial Cells. Mol. Neurobiol. 2016-01-05 [PMID: 26732598] (WB, Rat) Details: Fis1/TTC11 antibody was used for WB assays on lysates from rat brain endothelial cell line/RBE4 cells which were treated or not with taxol, nocadazole or cytochalasin B.	WB	Rat
Lim S Sex Differences in Cancer Cachexia and a Novel Mitochondrial Target for Cancer-Induced Muscle Wasting Thesis Jan 1 2022 (WB, Mouse)	WB	Mouse
Vijayan M, Reddy PH Reduced VDAC1, Maintained Mitochondrial Dynamics and Enhanced Mitochondrial Biogenesis in a Transgenic Tau Mouse Model of Alzheimer's Disease International journal of molecular sciences 2022-08-02 [PMID: 35955694] (WB, IF/IHC, Mouse) Details: WB dilution used 1:1000, IHC dilution used 1:102	WB, IF/IHC	Mouse
Wang X, Tang Y, Xie N et al. Salidroside, a phenyl ethanol glycoside from Rhodiola crenulata, orchestrates hypoxic mitochondrial dynamics homeostasis by stimulating Sirt1/p53/Drp1 signaling Journal of ethnopharmacology 2022-04-16 [PMID: 35439546] (WB, Mouse)	WB	Mouse
Lim S, Deaver JW, Rosa-Caldwell ME et al. Muscle miR-16 deletion results in impaired insulin sensitivity and contractile function in a sex-dependent manner American journal of physiology. Endocrinology and metabolism 2022-01-24 [PMID: 35068192]
Aloysius Dhivya M, Sulochana K, Devi S High glucose induced inflammation is inhibited by copper chelation via rescuing mitochondrial fusion protein 2 in retinal pigment epithelial cells Cellular Signalling 2022-01-01 [PMID: 34999205] (ICC/IF, Human)	ICC/IF	Human
Awasthi S, Hindle A, Sawant NA Et al. RALBP1 in Oxidative Stress and Mitochondrial Dysfunction in Alzheimer's Disease Cells 2021-11-10 [PMID: 34831336]
Lim S, Deaver JW, Rosa-Caldwell ME Et al. Development of metabolic and contractile alterations in development of cancer cachexia in female tumor-bearing mice Journal of applied physiology (Bethesda, Md. : 1985) 2021-11-11 [PMID: 34762526]
Ramya R, Bharathidevi SR Paraoxonase 2 protects against the CML mediated mitochondrial dysfunction through modulating JNK pathway in human retinal cells Biochimica et biophysica acta. General subjects 2021-10-25 [PMID: 34710487] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Rosa-Caldwell ME, Lim S, Haynie WS Et al. Mitochondrial aberrations during the progression of disuse atrophy differentially affect male and female mice Journal of cachexia, sarcopenia and muscle 2021-09-29 [PMID: 34585846] (WB, Mouse)	WB	Mouse
Fernandes T, Resende R, Silva D Et Al. Structural and Functional Alterations in Mitochondria-Associated Membranes (MAMs) and in Mitochondria Activate Stress Response Mechanisms in an In Vitro Model of Alzheimer s Disease Biomedicines 2021-07-24 [PMID: 34440085] (WB)	WB
Marques A, Resende R, Silva D et al. Mitochondrial Alterations in Fibroblasts of Early Stage Bipolar Disorder Patients Biomedicines 2021-05-07 [PMID: 34066918] (WB, Human)	WB	Human
Abid H, Ryan ZC, Delmotte P et al. Extramyocellular interleukin-6 influences skeletal muscle mitochondrial physiology through canonical JAK/STAT signaling pathways FASEB J. 2020-09-03 [PMID: 32885495]
Wongkitikamjorn W, Hosomichi J, Wada E et al. Gestational Intermittent Hypoxia Induces Mitochondrial Impairment in the Geniohyoid Muscle of Offspring Rats Cureus 2022-05-17 [PMID: 35600069] (Immunohistochemistry, Western Blot)	Immunohistochemistry, Western Blot
Gao QY, Zhang HF, Tao J et al. Mitochondrial Fission and Mitophagy Reciprocally Orchestrate Cardiac Fibroblasts Activation Frontiers in Cell and Developmental Biology 2021-01-21 [PMID: 33585469] (Western Blot)	Western Blot
Brown JL, Rosa-Caldwell ME, Lee DE et al. Mitochondrial degeneration precedes the development of muscle atrophy in progression of cancer cachexia in tumour-bearing mice J Cachexia Sarcopenia Muscle. 2017-12-01 [PMID: 28845591] (WB, Mouse)	WB	Mouse
El-Sikhry HE, Alsaleh N, Dakarapu R et al. Novel Roles of Epoxyeicosanoids in Regulating Cardiac Mitochondria. PLoS ONE. 2016-08-06 [PMID: 27494529] (WB, ELISA)	WB, ELISA
Santos D, Esteves AR, Silva DF et al. The Impact of Mitochondrial Fusion and Fission Modulation in Sporadic Parkinson's Disease. Mol Neurobiol. 2014-09-14 [PMID: 25218511] (WB, Human) Details: Fis1 /TTC11 antibody used for WB on cytosolic and mitochondrial preparations from CT and PD (sporadic Parkinson’s disease ) cybrids - the cytoplasmic hybrid cells prepared via the transfer of PD subject platelet mitochondria to mitochondrial DNA /mtDNA-depleted (rho0) human NT2 teratocarcinoma cells. Cybrids represents a model in which mitochondrial platelet mitochondria CXI defect persists and mitochondrial dysfunction is well characterized. Antibody also used for WB on PBMCs isolated from PD patients.	WB	Human
Santos RX, Correia SC, Alves MG et al. Mitochondrial quality control systems sustain brain mitochondrial bioenergetics in early stages of type 2 diabetes. Mol. Cell. Biochem. 2014-05-16 [PMID: 24833464] (WB, Rat) Details: Rat brain cortical tissue, Fig 2A.The Fis1 pAb was used at 1:750 and Fis1 was detected at ~17 kDa.	WB	Rat
Santos RX, Xcorreia SC, Alves MG et al. Insulin therapy modulates mitochondrial dynamics and biogenesis, autophagy and tau protein phosphorylation in the brain of type 1 diabetic animals. Biochim. Biophys. Acta 2014-04-18 [PMID: 24747740] (WB, Rat) Details: Mitochondrial extracts of brain cortex, Fig 3A. Fis1 was detected at ~17kDa.	WB	Rat
Liu R, Jin P, Liqunyu et al. Impaired mitochondrial dynamics and bioenergetics in diabetic skeletal muscle. PLoS ONE 2014-03-24 [PMID: 24658162] (WB, Mouse) Details: Skeletal muscle, Fig 7	WB	Mouse
Miller MW, Knaub LA, Olivera-Fragoso LF et al. Nitric oxide regulates vascular adaptive mitochondrial dynamics. Am J Physiol Heart Circ Physiol. 2013-06-15 [PMID: 23585138]
Wortmann SB, Vaz FM, Gardeitchik T et al. Mutations in the phospholipid remodeling gene SERAC1 impair mitochondrial function and intracellular cholesterol trafficking and cause dystonia and deafness. Nat Genet. 2012-06-10 [PMID: 22683713]
Wang X, Yan MH, Fujioka H et al. LRRK2 regulates mitochondrial dynamics and function through direct interaction with DLP1. Hum Mol Genet. 2012-05-01 [PMID: 22228096] (WB, Human) Details: WB: SH-SY5Y human neuroblastoma cells, Fig 4A.	WB	Human
Xu YF, Gendron TF, Zhang YJ et al. Wild-type human TDP-43 expression causes TDP-43 phosphorylation, mitochondrial aggregation, motor deficits, and early mortality in transgenic mice. J Neurosci. 2010-08-11 [PMID: 20702714] (WB) Details: WB: Mouse brain cells, Fig 5E.	WB
Wang X, Su B, Lee HG et al. Impaired balance of mitochondrial fission and fusion in Alzheimer's disease. J Neurosci. 2009-07-15 [PMID: 19605646] (WB, IHC-P, Human) Details: 1. IHC (p), Fig 1C,D: Hippocampus from Alzheimer's and normal human brain. Tissues were fixed in methacarn (methanol:chloroform:acetic acid, 6:3:1) and embedded in paraffin. See publication for additional details 2. WB, Fig 1A: Hippocampus from Alzheimer'	WB, IHC-P	Human
Wang X, Su B, Siedlak SL et al. Amyloid-{beta} overproduction causes abnormal mitochondrial dynamics via differential modulation of mitochondrial fission/fusion proteins. Proc Natl Acad Sci U S A 2008-12-09 [PMID: 19050078] (WB, Human)	WB	Human
Duvezin-Caubet S, Jagasia R, Wagener J et al. Proteolytic processing of OPA1 links mitochondrial dysfunction to alterations in mitochondrial morphology. J Biol Chem. 2006-12-08 [PMID: 17003040]
Beatriz M, VilaCa R, Anjo S et al. Defective mitochondria lysosomal axis enhances the release of extracellular vesicles containing mitochondrial DNA and proteins in Huntington's disease Journal of Extracellular Biology 2022-10-01 (WB, Human) Details: Dilution used in WB 1:1000	WB	Human
Show All 32 Publications. Collapse Publications.

Array NB100-56646

• TTC11 Antibodies
• TTC11 ELISA Kits
• TTC11 Lysate
• TTC11 Proteins