![Western Blot: MED12 Antibody [NB100-2357] - Components from all modules of Mediator are found in TAP-wtICP4 samples. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses at 6 hpi. Results of the Western blot analysis for components of the head (Med6), middle (Med4 and Med1), tail (Med23), kinase (cdk8, Med12, and Med13), and the Med26 protein are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MED12-Antibody-Western-Blot-NB100-2357-img0015.jpg "Western Blot: MED12 Antibody [NB100-2357] - Components from all modules of Mediator are found in TAP-wtICP4 samples. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses at 6 hpi. Results of the Western blot analysis for components of the head (Med6), middle (Med4 and Med1), tail (Med23), kinase (cdk8, Med12, and Med13), and the Med26 protein are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Pm, PmSpecies Glossary |
| Applications | WB, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 2150 and the C-terminus (residue 2212) of human Mediator of RNA Polymerase II Transcription, subunit 12 (NP_005111.1). |
| Predicted Species | Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MED12 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31089260). |
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| Theoretical MW | 243 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry Prohibitin Antibody [Unconjugated]](https://images.novusbio.com/images2/Prohibitin_AF3470_Immunocytochemistry__Immunofluorescence_20123.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for MED12 Antibody (NB100-2357)Find related products by research area.
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![Immunohistochemistry: MED12 Antibody [NB100-2357] - Representative examples of immunohistochemically stained sections positive for MED12 (A, B and C) in tumor specimens. Examples are from the same patients, respectively. A. TS = 5, B. TS = 6, C. TS = 6 (x20 original magnification [A, B and C]. Scale bars represent 100 um. TS, total score = intensity score (0-3) plus proportion score (0-3). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/14410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-NB100-2357-img0014.jpg "Immunohistochemistry: MED12 Antibody [NB100-2357] - Representative examples of immunohistochemically stained sections positive for MED12 (A, B and C) in tumor specimens. Examples are from the same patients, respectively. A. TS = 5, B. TS = 6, C. TS = 6 (x20 original magnification [A, B and C]. Scale bars represent 100 um. TS, total score = intensity score (0-3) plus proportion score (0-3). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/14410), licensed under a CC-BY license.")
![Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957332.jpg "Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from HeLa, A-549, GaMG, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957333.jpg "Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from HeLa, A-549, GaMG, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957330.jpg "Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for IP at 6 ug per reaction. Med12 was also immunoprecipitated by a previous lot of this antibody (NB100-2357 lot 2) and a second antibody against a different epitope of Med12 (NBP2-80138). For blotting immunoprecipitated Med12, NB100-2357 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957334.jpg "Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for IP at 6 ug per reaction. Med12 was also immunoprecipitated by a previous lot of this antibody (NB100-2357 lot 2) and a second antibody against a different epitope of Med12 (NBP2-80138). For blotting immunoprecipitated Med12, NB100-2357 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957331.jpg "Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")