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MED12 Antibody [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Pm, PmSpecies Glossary
Applications WB, IHC, ChIP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

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MED12 Antibody [Janelia Fluor® 549] Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2150 and the C-terminus (residue 2212) of human Mediator of RNA Polymerase II Transcription, subunit 12 (NP_005111.1).
Predicted Species
Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MED12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 24147125).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MED12 Antibody [Janelia Fluor® 549]

  • ARC240
  • CAG repeat protein 45
  • CAGH45trinucleotide repeat containing 11 (THR-associated protein, 230kDa subunit)
  • HOPAFGS1
  • KIAA0192FG syndrome 1
  • mediator complex subunit 12OPA-containing protein
  • OKSsubunit 12 homolog
  • thyroid hormone receptor-associated protein, 230 kDa subunit
  • Trap230
  • TRAP230mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae)
  • trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit)
  • Trinucleotide repeat-containing gene 11 protein

Background

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional MED12 Products

Array NB100-2357JF549

Research Areas for MED12 Antibody (NB100-2357JF549)

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Blogs on MED12

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Bioinformatics

Gene Symbol MED12