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PARL Antibody

Images

 
Orthogonal Strategies: Western Blot: PARL Antibody [NBP1-80878] - Analysis in human cell line CACO-2 and human cell line HeLa.
Immunocytochemistry/ Immunofluorescence: PARL Antibody [NBP1-80878] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: PARL Antibody [NBP1-80878] - Staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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PARL Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ESLKSRVQSYFDGIKADWLDSIRPQKEGDFRKEINKWWNNLSDGQR
Predicted Species
Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PARL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PARL Recombinant Protein Antigen (NBP1-80878PEP)
Publications
Read Publications using NBP1-80878.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PARL Antibody

  • EC 3.4.21.105
  • mitochondrial
  • PARL
  • presenilin associated, rhomboid-like
  • PRO2207
  • PSARL1
  • RHBDS1
  • rhomboid 7 homolog 1

Background

PARL encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PARL Antibody (NBP1-80878)(2)


Showing Publications 1 - 2 of 2.
Publications using NBP1-80878 Applications Species
Gonciarz R, Jiang H, Hugelshofter C et al. In vivo Bioluminescence Imaging of Labile Iron in Xenograft Models and Liver Using FeAl-1, an Iron-Activatable Form of D-Luciferin SSRN Electronic Journal 2022-11-20
Subramaniam, N, N The Contribution of LncRNAs to Tip-Stalk Patterning in the Vascular Endothelium Thesis 2022-01-01

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PARL Antibody (NBP1-80878) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PARL
Entrez
Uniprot