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TSPAN7/TM4SF2 Antibody (482618) [Unconjugated]

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HEK293 human embryonic kidney cell line either transfected with (A) human TSPAN7 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN7 Monoclonal Antibody (Catalog # MAB9179) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line either transfected with (A) human TSPAN7 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN7 Monoclonal Antibody (Catalog # MAB9179) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
482618
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB9179U-100UG
Alexa Fluor 405
FAB9179V-100UG
Alexa Fluor 488
FAB9179G-100UG
Alexa Fluor 532
FAB9179X
Alexa Fluor 594
FAB9179T-100UG
Alexa Fluor 647
FAB9179R-025
Alexa Fluor 700
FAB9179N-100UG
Alexa Fluor 750
FAB9179S-100UG
Allophycocyanin
FAB9179A
Allophycocyanin/Cy7
FAB9179APCCY7
Biotin
FAB9179B
CoraFluor 1
FAB9179CL1
DyLight 350
FAB9179D
DyLight 405
FAB9179E
DyLight 488
FAB9179K
DyLight 550
FAB9179L
DyLight 594
FAB9179M
DyLight 650
FAB9179W
DyLight 680
FAB9179Y
DyLight 755
FAB9179Z
FITC
FAB9179F
HRP
FAB9179H
Janelia Fluor 525
FAB9179JF525
Janelia Fluor 549
FAB9179I
Janelia Fluor 585
FAB9179JF585
Janelia Fluor 635
FAB9179JF635
Janelia Fluor 646
FAB9179J
Janelia Fluor 669
FAB9179JF669
PE
FAB9179P
PE/Atto594
FAB9179PEATT594
PE/Cy5.5
FAB9179PECY55
PE/Cy7
FAB9179PECY7
PerCP
FAB9179C
mFluor Violet 450 SE
FAB9179MFV450
mFluor Violet 500 SE
FAB9179MFV500
mFluor Violet 610 SE
FAB9179MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB9179-SP
Carrier Free
FAB9179R-025
Carrier Free
FAB9179N-100UG
Carrier Free
FAB9179G-100UG
Carrier Free
FAB9179V-100UG
Carrier Free
FAB9179U-100UG
Carrier Free
FAB9179T-100UG
Carrier Free
FAB9179S-100UG
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TSPAN7/TM4SF2 Antibody (482618) [Unconjugated] Summary

Immunogen
HEK293 human embryonic kidney cell line transfected with human TSPAN7
Accession # P41732
Specificity
Stains human TSPAN7 transfectants but not irrelevant transfectants in flow cytometry.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN7/TM4SF2 Antibody (482618) [Unconjugated]

  • A15
  • A15CCG-B7
  • CCG-B7
  • CD231 antigen
  • CD231
  • Cell surface glycoprotein A15
  • DXS1692E
  • Membrane component chromosome X surface marker 1
  • mental retardation, X-linked 58
  • MRX58
  • MXS1
  • MXS1T-cell acute lymphoblastic leukemia associated antigen 1
  • TALLA-1
  • T-cell acute lymphoblastic leukemia-associated antigen 1
  • tetraspanin 7
  • tetraspanin-7
  • TM4SF2
  • TM4SF2b
  • TM4SF2tetraspanin protein
  • transmembrane 4 superfamily 2b
  • Transmembrane 4 superfamily member 2X chromosome, surface marker 1
  • transmembrane protein A15
  • TSPAN7
  • tspan-7

Background

Tetraspanin-7 (aka TSPAN7,  TALLA-1, TM4SF2 and CD231) is a 249 aminoacids (aa) multi-pass membrane protein.  Tetraspanin proteins regulate morphology, signaling and trafficking processes by interaction and association with proteins in tetraspanin enriched microdomains (TEMs). It has been proposed that tetraspanins interact with integrins to affect cell migration, probably by modulation or compartmentalization of integrin signaling. TSPAN7 has been found to be upregulated in myeloma development and described as a prognostic marker of renal cell carcinoma. Mutations in TSPAN7 are also implicated in some forms of X-linked intellectual disability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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