Name: TNF RI/TNFRSF1A Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF225
Price: 509 USD

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Flow, CyTOF-ready, Func, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF225

Immunogen	E. coli-derived recombinant human sTNF RI/TNFRSF1A Accession # NP_001056
Specificity	Detects human sTNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse TNF sRI and recombinant rat TNF sRI is observed. This anti-human TNF RI antibody can interact with the cell surface 55 kDa human TNF RI and exhibits TNF agonist activities on the A549 human lung carcinoma cell line. This antibody also exhibits TNF agonist activities on the L‑929 mouse fibroblast cell line, suggesting that it has cross-species activity with mouse cell surface TNF RI.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	TNFRSF1A
Purity Statement	Antigen Affinity-purified
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

TNF RI/TNFRSF1A Antibody [Unconjugated]

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Publications for TNF RI/TNFRSF1A Antibody (AF225)(14)

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Bioinformatics

Publications using AF225	Applications	Species
Nam, YW;Shin, JH;Kim, S;Hwang, CH;Lee, CS;Hwang, G;Kim, HR;Roe, JS;Song, J; EGFR inhibits TNF-?-mediated pathway by phosphorylating TNFR1 at tyrosine 360 and 401 Cell death and differentiation 2024-05-24 [PMID: 38789573] (Immunocytochemistry, Human)	Immunocytochemistry	Human
Miguel Carracedo, Elke Ericson, Rasmus Ågren, Anna Forslöw, Katja Madeyski-Bengtson, Anna Svensson et al. APOL1 promotes endothelial cell activation beyond the glomerulus iScience 2023-06-16 [PMID: 37250770]
D McMillan, C Martinez-F, J Porter, D Fox, R Davis, P Mori, T Ceska, B Carrington, A Lawson, T Bourne, J O'Connell Structural insights into the disruption of TNF-TNFR1 signalling by small molecules stabilising a distorted TNF Nature Communications, 2021-01-25;12(1):582. 2021-01-25 [PMID: 33495441] (Functional Assay, Human)	Functional Assay	Human
A Rodriguez-, P Sharma, M Poussin, AC Boesteanu, NG Minutolo, SB Gitto, DK Omran, MK Robinson, GP Adams, F Simpkins, DJ Powell CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic Malignancies Mol. Ther., 2019-12-06;0(0):. 2019-12-06 [PMID: 31870622] (Neutralization, Human)	Neutralization	Human
J O'Connell, J Porter, B Kroeplien, T Norman, S Rapecki, R Davis, D McMillan, T Arakaki, A Burgin, D Fox Iii, T Ceska, F Lecomte, A Maloney, A Vugler, B Carrington, BP Cossins, T Bourne, A Lawson Small molecules that inhibit TNF signalling by stabilising an asymmetric form of the trimer Nat Commun, 2019-12-19;10(1):5795. 2019-12-19 [PMID: 31857588] (Neutralization, Human)	Neutralization	Human
Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine. PLoS ONE, 2015-12-02;10(12):e0143224. 2015-12-02 [PMID: 26630176] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Dariusz Szukiewicz, Malgorzata Wojciechowska, Anna Bilska, Aleksandra Stangret, Grzegorz Szewczyk, Tarun Kumar Mittal et al. Aspirin Action in Endothelial Cells: Different Patterns of Response Between Chemokine CX3CL1/CX3CR1 and TNF-alpha /TNFR1 Signaling Pathways Cardiovascular Drugs and Therapy 2015-06-01 [PMID: 25959742]
Wang Y, Tan B, Mu R, Chang Y, Wu M, Tu H, Zhang Y, Guo S, Qin X, Li T, Li W, Li A, Zhang X, Li H Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor kappaB (NF-kappaB) signaling. J Biol Chem, 2015-02-13;290(16):10395-405. 2015-02-13 [PMID: 25681446] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Jung-Min Choi, Sushil Devkota, Young Hoon Sung, Han-Woong Lee EI24 regulates epithelial-to-mesenchymal transition and tumor progression by suppressing TRAF2-mediated NF-kappa B activity Oncotarget 2013-12-31 [PMID: 24280371]
Shuo Yang, Bingwei Wang, Lisa S. Tang, Jakub Siednienko, John J. Callanan, Paul N. Moynagh Pellino3 targets RIP1 and regulates the pro-apoptotic effects of TNF-alpha Nature Communications 2013-10-11 [PMID: 24113711]
Isabelle Jéru, Serge Charmion, Emmanuelle Cochet, Bruno Copin, Philippe Duquesnoy, Maria Teresa Mitjavila Garcia et al. Involvement of the Same TNFR1 Residue in Mendelian and Multifactorial Inflammatory Disorders PLoS ONE 2013-07-24 [PMID: 23894535]
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012-02-17;378(1):72-80. 2012-02-17 [PMID: 22366959] (ELISA Development, Human)	ELISA Development	Human
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 2007-03-30;52(10):2775-83. 2007-03-30 [PMID: 17394066] (ICC, Human)	ICC	Human
Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression. Atherosclerosis, 2006-07-20;192(1):25-32. 2006-07-20 [PMID: 16857205] (IHC-P, Porcine)	IHC-P	Porcine
Show All 14 Publications. Collapse Publications.

Catalog# & Formulation	Size	Price
Carrier Free AF225-SP

Gene Symbol	TNFRSF1A
Uniprot	NP_001056()