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Human Synaptopodin ELISA Kit (Colorimetric)

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ELISA: Human Synaptopodin ELISA Kit (Colorimetric) [NBP2-80297] - Samples were spiked with high concentrations of Human Synaptopodin and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Human Synaptopodin ELISA Kit (Colorimetric) [NBP2-80297] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL

Order Details

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Human Synaptopodin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human SYNPO in samples. No significant cross-reactivity or interference between Human SYNPO and analogues was observed.
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.13%
Intra-Assay
CV% < 4.09%
Spike Recovery
86-110%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
SYNPO

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25X)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Synaptopodin ELISA Kit (Colorimetric)

  • KIAA1029
  • Synaptopodin
  • SYNPO

Background

Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SYNPO
Uniprot