Home » STAT5a/b » STAT5a/b Primary Antibodies » STAT5a/b [p Tyr694, p Tyr699] Antibody (941212) [Unconjugated]

STAT5a/b [p Tyr694, p Tyr699] Antibody (941212) [Unconjugated]

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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB4190) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT5a/b (Y694/Y699) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for ...read more
STAT5a/b phosphorylated at Y694/Y699 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB4190) at 0.3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
941212
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB4190-SP
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STAT5a/b [p Tyr694, p Tyr699] Antibody (941212) [Unconjugated] Summary

Immunogen
Phosphopeptide containing human STAT5b Y699 site (amino acid sequence of this peptide is identical to a corresponding region of human STAT5a containing Y694).
Accession # P51692
Modification
p Tyr694, p Tyr699
Specificity
Detects human STAT5a/b when phosphorylated at Y694/Y699 in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 0.3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT5a/b [p Tyr694, p Tyr699] Antibody (941212) [Unconjugated]

  • MGF
  • STAT5
  • STAT5A signal transducer and activator of transcription 5A
  • STAT5a/b

Background

Signal Transduction and Activator of Transcription 5B (STAT5b) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL-22, IL-6, IFN alpha ). Upon cytokine activation, STAT5b is phosphorylated and translocates to the nucleus where it binds to the DNA consensus site TTCN3GAA and activates transcription of specific genes. STAT5b shares 93% amino acid identity with STAT5a. STAT5b is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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