Home » SMURF1 » SMURF1 Antibodies » SMURF1 Antibody (530131) [Unconjugated]

SMURF1 Antibody (530131) [Unconjugated]

 
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMURF1 Monoclonal Antibody (Catalog # MAB9507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SMURF1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMURF1 Monoclonal Antibody (Catalog # MAB9507) followed by HRP-conjugated ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SMURF1 at approximately 82 kDa (as indicated) using 10 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
530131
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB9507-SP
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SMURF1 Antibody (530131) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SMURF1 
Met496-Glu757
Accession # Q9HCE7
Specificity
Detects human SMURF1 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMURF1 Antibody (530131) [Unconjugated]

  • E3 ubiquitin ligase SMURF1
  • EC 6.3.2
  • EC 6.3.2.-
  • hSMURF1
  • KIAA1625
  • KIAA1625E3 ubiquitin-protein ligase SMURF1
  • SMAD specific E3 ubiquitin protein ligase 1
  • SMAD ubiquitination regulatory factor 1
  • Smad-specific E3 ubiquitin ligase 1
  • SMAD-specific E3 ubiquitin-protein ligase 1
  • SMURF1

Background

Smurf-1 is a 757 amino acids E3 ubiquitin-protein ligase encoded by the SMURF1 gene. Smurf-1 ubiquitinates receptor regulated SMAD proteins acting as a negative regulator of BMP signaling pathway, and it has been shown to inhibit osteoblast differentiation by targeting Runx2, the master regulator of osteoblast differentiation, for degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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