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SKI Antibody - BSA Free

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Western Blot: SKI Antibody [NBP1-77305] - Analysis in HeLa cell lysate with antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: SKI Antibody [NBP1-77305] - Analysis in HeLa cell lysate with antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: SKI Antibody - BSA Free [NBP1-77305] - Immunofluorescence of SKI in human kidney tissue with SKI antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Ch, EqSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-77305-0.025mg
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SKI Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski. The immunogen is located within the first 50 amino acids of Ski. Amino Acid Squence: CFQPHPGLQKTLEQ
Predicted Species
Chicken (100%), Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SKI
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Western Blot 1-2 ug/mL
Control Peptide
SKI Peptide (NBP1-77305PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for SKI Antibody - BSA Free

  • C-ski
  • Proto-oncogene c-Ski
  • ski oncogene
  • ski oncoprotein
  • SKI
  • SKV
  • v-ski avian sarcoma viral oncogene homolog
  • v-ski sarcoma viral oncogene homolog (avian)

Background

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SKI Antibody - BSA
Free
NBP1-77305
SKI Antibody - BSA Free
Species: Hu, Ch, Eq
Applications: WB, ELISA, ICC/IF

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SKI
Uniprot