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Chromogranin B Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Analysis in human adrenal gland and liver tissues using NBP1-80782 antibody. Corresponding CHGB RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human adrenal gland, colon, liver and pancreas using Anti-CHGB antibody NBP1-80782 (A) shows similar ...read more
Western Blot: Chromogranin B Antibody [NBP1-80782] - Analysis in human cell line SH-SY5Y.
Immunocytochemistry/ Immunofluorescence: Chromogranin B Antibody [NBP1-80782] - Staining of human cell line SH-SY5Y shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human colon shows strong cytoplasmic positivity in enteroendocrine cells.
Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human colon shows strong cytoplasmic positivity in enteroendocrine cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Chromogranin B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KQYSSHHTAEKRKRLGELFNPYYDPLQWKSSHFERRDNMNDNFLEGEEENELTLNEKNFFPEYNYDWWEKKPFSEDVNWGYEKRNLARVPKLDLKRQYDRVAQLDQLLHYRKKSAEFPDFYDS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHGB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Chromogranin B Protein (NBP1-80782PEP)
Publications
Read Publications using
NBP1-80782 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Chromogranin B Antibody

  • CgB
  • CHGB
  • chromogranin B (secretogranin 1)
  • Chromogranin B
  • Chromogranin-B
  • SCG1
  • SCG1sgI
  • secretogranin B
  • Secretogranin I
  • secretogranin-1
  • SgI

Background

The Chromogranin B gene encodes a tyrosine-sulfated secretory protein abundant in peptidergic endocrine cells and neurons. Thisprotein may serve as a precursor for regulatory peptides. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Chromogranin B Antibody (NBP1-80782)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: WB.


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WB
(2)
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Mouse
(1)
Rat
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Chromogranin B Antibody (NBP1-80782) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHGB
Entrez
Uniprot