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Serpin A6/Cortisol Binding Globulin Antibody

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Serpin A6 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Serpin A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4065) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Serpin A6/Cortisol Binding Globulin Antibody Summary

Specificity
Detects mouse Serpin A6 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat Serpin A6 is observed and less than 1% cross-reactivity with recombinant human (rh) Serpin A1 and rhSerpin A5 is observed. In Western blots, less than 5% cross-reactivity with recombinant human Serpin A4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SERPINA6
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A6/Cortisol Binding Globulin Antibody

  • antitrypsin), member 6
  • CBG
  • CBGcorticosteroid-binding globulin
  • corticosteroid binding globulin
  • member 6
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A6
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
  • transcortin

Background

Serpin A6 (also known as corticosteroid-binding globulin/CBG and Transcortin) is a 50‑60 kDa, secreted glycoprotein member of the serpin family of protease inhibitors. It is produced by hepatocytes and transports > 90% of circulating cortisol. Although Serpin A6 is a serpin family member, it shows no protease inhibition. Mature mouse Serpin A6 is 375 amino acids (aa) in length. Over aa 23‑397, mouse Serpin A6 shares 78% and 57% aa sequence identity with rat and human Serpin A6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SERPINA6
Uniprot