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Siglec-3/CD33 Antibody (6C5/2) [DyLight 550]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
6C5/2
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

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Siglec-3/CD33 Antibody (6C5/2) [DyLight 550] Summary

Immunogen
Recombinant human Siglec‑3/CD33
Extracellular domain
Specificity
Detects human Siglec‑3/CD33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Siglec-2, -5, -7, or -9 is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD33
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Siglec-3/CD33 Antibody (6C5/2) [DyLight 550]

  • CD33 antigen (gp67)
  • CD33 antigen
  • CD33 molecule
  • CD33
  • FLJ00391
  • gp67
  • myeloid cell surface antigen CD33
  • p67
  • sialic acid binding Ig-like lectin 3
  • Sialic acid-binding Ig-like lectin 3
  • Siglec3
  • Siglec-3
  • SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Siglec-3/CD33 Antibody (FAB1137L) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD33