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Siglec-3/CD33 Antibody (6C5/2) [DyLight 755]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
6C5/2
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1137U-100UG
Alexa Fluor 405
FAB1137V-100UG
Alexa Fluor 488
FAB1137G-100UG
Alexa Fluor 532
FAB1137X
Alexa Fluor 594
FAB1137T-100UG
Alexa Fluor 647
FAB1137R-100UG
Alexa Fluor 700
FAB1137N-100UG
Alexa Fluor 750
FAB1137S-100UG
Allophycocyanin
FAB1137A-025
Allophycocyanin
FAB1137A-100
Allophycocyanin/Cy7
FAB1137APCCY7
Biotin
FAB1137B
CoraFluor 1
FAB1137CL1
DyLight 350
FAB1137D
DyLight 405
FAB1137E
DyLight 488
FAB1137K
DyLight 550
FAB1137L
DyLight 594
FAB1137M
DyLight 650
FAB1137W
DyLight 680
FAB1137Y
HRP
FAB1137H
Janelia Fluor 525
FAB1137JF525
Janelia Fluor 549
FAB1137I
Janelia Fluor 585
FAB1137JF585
Janelia Fluor 635
FAB1137JF635
Janelia Fluor 646
FAB1137J
Janelia Fluor 669
FAB1137JF669
PE/Atto594
FAB1137PEATT594
PE/Cy5.5
FAB1137PECY55
PE/Cy7
FAB1137PECY7
PerCP
FAB1137C
Phycoerythrin
FAB1137P-100
Phycoerythrin
FAB1137P-025
Unconjugated
MAB1137-100
mFluor Violet 450 SE
FAB1137MFV450
mFluor Violet 500 SE
FAB1137MFV500
mFluor Violet 610 SE
FAB1137MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1137-100
Carrier Free
FAB1137P-025
Carrier Free
FAB1137P-100
Carrier Free
FAB1137A-025
Carrier Free
FAB1137A-100
Carrier Free
MAB1137-SP
Carrier Free
MAB1137-100
Carrier Free
MAB1137-500
Carrier Free
FAB1137V-100UG
Carrier Free
FAB1137U-100UG
Carrier Free
FAB1137T-100UG
Carrier Free
FAB1137S-100UG
Carrier Free
FAB1137R-100UG
Carrier Free
FAB1137N-100UG
Carrier Free
FAB1137G-100UG
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Siglec-3/CD33 Antibody (6C5/2) [DyLight 755] Summary

Immunogen
Recombinant human Siglec‑3/CD33
Extracellular domain
Specificity
Detects human Siglec‑3/CD33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Siglec-2, -5, -7, or -9 is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD33
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Siglec-3/CD33 Antibody (6C5/2) [DyLight 755]

  • CD33 antigen (gp67)
  • CD33 antigen
  • CD33 molecule
  • CD33
  • FLJ00391
  • gp67
  • myeloid cell surface antigen CD33
  • p67
  • sialic acid binding Ig-like lectin 3
  • Sialic acid-binding Ig-like lectin 3
  • Siglec3
  • Siglec-3
  • SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD33