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SA1 Antibody

Images

 
Western Blot: SA1 Antibody [NB100-298] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-298] - HeLa cells that were extracted for 5 min at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, InSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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SA1 Antibody Summary

Immunogen
A 36 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 1 encoded in part by exons 5 and 6 (LocusLink ID 10274).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STAG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2000
  • Immunoprecipitation
  • Western Blot 1:1000-1:10000
Application Notes
NB 100-298 may be used for Western Blot where a band at ~145 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. Suggested working dilutions: * Western Blot 1:1,000-1:10,000 (ECL) Immunocytochemistry 1:500-1:2,000 Immunoprecipitation Not Done *The investigator should determine the optimal working dilution for a specific application.
Control
HeLa Nuclear Cell Lysate
Publications
Read Publications using
NB100-298 in the following applications:

  • WB
    2 publications

Reactivity Notes

Invetebrate reactivity reported in (PMID: 23874961).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SA1 Antibody

  • cohesin subunit SA-1
  • nuclear protein stromal antigen 1
  • SA1
  • stromal antigen 1

Background

SA 1 is expressed as an approximately 155 kD protein that assembles with the cohesin proteins, SMC1, SMC3 and SCC1. The cohesin complex also coprecipitates with PDS5, but in a less stable way than the proteins in the cohesin assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SA1 Antibody (NB100-298)(3)

We have publications tested in 1 confirmed species: Invertebrate.

We have publications tested in 1 application: WB.


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WB
(2)
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Filter By Species
Invertebrate
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SA1 Antibody (NB100-298) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on SA1

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Bioinformatics

Gene Symbol STAG1