I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?

Name: SMC1 Antibody
Brand: Novus Biologicals
SKU: NB100-204
Price: 449 USD

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Accepted Answer

My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, BvSpecies Glossary
Applications	WB, Simple Western, ELISA, ICC/IF, IHC, IP
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-204AF350

Alexa Fluor 405 NB100-204AF405

Alexa Fluor 488 NB100-204AF488

Alexa Fluor 532 NB100-204AF532

Alexa Fluor 594 NB100-204AF594

Alexa Fluor 647 NB100-204AF647

Alexa Fluor 700 NB100-204AF700

Alexa Fluor 750 NB100-204AF750

Allophycocyanin NB100-204APC

Biotin NB100-204B

CoraFluor 1 NB100-204CL1

DyLight 350 NB100-204UV

DyLight 405 NB100-204V

DyLight 488 NB100-204G

DyLight 550 NB100-204R

DyLight 594 NB100-204DL594

DyLight 650 NB100-204C

DyLight 680 NB100-204FR

DyLight 755 NB100-204IR

FITC NB100-204F

HRP NB100-204H

Janelia Fluor 525 NB100-204JF525

Janelia Fluor 549 NB100-204JF549

Janelia Fluor 646 NB100-204JF646

Janelia Fluor 669 NB100-204JF669

PE NB100-204PE

PerCP NB100-204PCP

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-204AF350
Alexa Fluor 405	NB100-204AF405
Alexa Fluor 488	NB100-204AF488
Alexa Fluor 532	NB100-204AF532
Alexa Fluor 594	NB100-204AF594
Alexa Fluor 647	NB100-204AF647
Alexa Fluor 700	NB100-204AF700
Alexa Fluor 750	NB100-204AF750
Allophycocyanin	NB100-204APC
Biotin	NB100-204B
CoraFluor 1	NB100-204CL1
DyLight 350	NB100-204UV
DyLight 405	NB100-204V
DyLight 488	NB100-204G
DyLight 550	NB100-204R
DyLight 594	NB100-204DL594
DyLight 650	NB100-204C
DyLight 680	NB100-204FR
DyLight 755	NB100-204IR
FITC	NB100-204F
HRP	NB100-204H
Janelia Fluor 525	NB100-204JF525
Janelia Fluor 549	NB100-204JF549
Janelia Fluor 646	NB100-204JF646
Janelia Fluor 669	NB100-204JF669
PE	NB100-204PE
PerCP	NB100-204PCP

Description	This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-224, NB100-206
Immunogen	The immunogen recognized by this antibody maps to a region between residue 1175 and the C-terminus of human Structural Maintenance of Chromosomes 1 using the numbering given in entry NP_006297.2
Localization	Nuclear
Predicted Species	Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	SMC1A
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

SMC1 Antibody

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Publications for SMC1 Antibody (NB100-204)(36)

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Storage	Store at 4C. Do not freeze.
Buffer	Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative	0.09% Sodium Azide
Concentration	1.0 mg/ml
Purity	Immunogen affinity purified

Publications using NB100-204	Applications	Species
Guo Y, Chomiak AA, Hong Y et al. Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration iScience 2022-07-15 [PMID: 35754718] (WB, Mouse)	WB	Mouse
Yi F, Zhang Y, Wang Z et al. The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis Science advances 2021-02-01 [PMID: 33627431] (IP, WB, Mouse)	IP, WB	Mouse
Wang C, Jette N, Moussienko D et al. ATM-Deficient Colorectal Cancer Cells Are Sensitive to the PARP Inhibitor Olaparib. Transl Oncol. 2017-02-06 [PMID: 28182994] (WB, Human)	WB	Human
Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015-03-27 [PMID: 25820262] (WB, Mouse)	WB	Mouse
Houlihan SL, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. Elife. 2014-09-23 [PMID: 25245017] (IHC-P, Mouse)	IHC-P	Mouse
A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200C>A with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity. Nakamura K, Fike F, Haghayegh S Mol Genet Genomic Med 2014-02-02 [PMID: 25077176] (WB, Human)	WB	Human
Arita , Yoh, Nakaoka , Yoshikaz, Matsunaga , Taichi, Kidoya , Hiroyasu, Yamamizu , Kohei, Arima , Yuichiro, Kataoka-Hashimoto , Takahiro, Ikeoka , Kuniyasu, Yasui , Taku, Masaki , Takeshi, Yamamoto , Kaori, Higuchi , Kaori, Park , Jin-Sung, Shirai , Manabu, Nishiyama , Koichi, Yamagishi , Hiroyuki, Otsu , Kinya, Kurihara , Hiroki, Minami , Takashi, Yamauchi-Takihara , Keiko, Koh , Gou Y, Mochizuki , Naoki, Takakura , Nobuyuki, Sakata , Yasushi, Yamashita , Jun K, Komuro , Issei Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart. Nat Commun, 2014-07-29;5(0):4552. 2014-07-29 [PMID: 25072663]
Puccini J, Shalini S, Voss AK et al. Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice. Proc Natl Acad Sci U S A 2013-12-03 [PMID: 24248351] (WB, Mouse)	WB	Mouse
Nayler S, Gatei M, Kozlov S et al. Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells: Induced Pluripotent Stem Cells from Ataxia-Telangiectasia Recapitulate the Cellular Phenotype Stem Cells Transl Med 2012-12-01 [PMID: 23197857]
Daniel JA, Pellegrini M, Lee BS et al. Loss of ATM kinase activity leads to embryonic lethality in mice J Cell Biol 2012-08-06 [PMID: 22869595] (WB, Mouse)	WB	Mouse
Gatei M, Jakob B, Chen P et al. ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control J Biol Chem 2011-09-09 [PMID: 21757780] (WB, Human)	WB	Human
Devgan SS, Sanal O, Doil C et al. Homozygous deficiency of ubiquitin-ligase ring-finger protein RNF168 mimics the radiosensitivity syndrome of ataxia-telangiectasia. Cell Death Differ;18(9):1500-1506. 2011-09-01 [PMID: 21394101]
Rajesh C, Baker DK, Pierce AJ, Pittman DL. The splicing-factor related protein SFPQ/PSF interacts with RAD51D and is necessary for homology-directed repair and sister chromatid cohesion Nucleic Acids Res 2011-01-01 [PMID: 20813759] (WB, Mouse)	WB	Mouse
Hu H, Du L, Nagabayashi G et al. ATM is down-regulated by N-Myc-regulated microRNA-421. Proc Natl Acad Sci USA 2010-01-01 [PMID: 20080624] (ELISA, WB, Human)	ELISA, WB	Human
Yoshizawa-Sugata N, Masai H. Roles of human AND-1 in chromosome transactions in S phase. J Biol Chem 2009-07-01 [PMID: 19439411]
Waltes R, Kalb R, Gatei M et al. Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder. Am J Hum Genet. 2009-05-01 [PMID: 19409520] (IP, WB, Human)	IP, WB	Human
Morales, M et al. The Rad50s allele promotes ATM-dependent DNA damage responses suppresses ATM deficiency: implications for the Mre11 complex as a DNA damage sensor. Genes Dev 19:3043-3054. 2005-01-01 [PMID: 16857186]
Lu S, Shen K, Wang Y et al. Atm-haploinsufficiency enhances susceptibility to carcinogen-induced mammary tumors. Carcinogenesis 27:848-855. 2006-01-01 [PMID: 16400190]
Adamson, AW et al. Methylator-induced, mismatch repair-dependent G2 arrest is activated through Chk1 Chk2. Mol Biol Cell 16(3):1513-1526. 2005-01-01 [PMID: 15647386] (WB, Human)	WB	Human
Escobar J, Frank JW, Suryawan A et al. Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation. Am J Physiol Endocrinol Metab 2005-05-01 [PMID: 15644455] (IP, WB, Human)	IP, WB	Human
Musio A, Montagna C, Mariani T et al. SMC1 involvement in fragile site expression. Hum Mol Genet 2005-02-01 [PMID: 15640246]
Hickson I, Zhao Y, Richardson CJ et al. Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM. Cancer Res 2004-12-01 [PMID: 15604286]
Kim, WJ et al. The monofunctional alkylating agent N-methyl-N' nitro-N-nitrosoguanidine triggers apoptosis through p53-dependent independent pathways. Toxicol-Appl Pharmacol 202(1):84-98. 2005-01-01 [PMID: 15589979] (Human)		Human
Kurz, E et al. Doxorubicin Activates ATM-dependent Phosphorylation of Multiple Downstream Targets in Part through the Generation of Reactive Oxygen Species. The Journal of Biological Chemistry 279(51): 53272-53281. 2004-01-01 [PMID: 15489221]
Jowsey PA, Doherty AJ, Rouse J et al. Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. J Biol Chem 2004-12-01 [PMID: 15456759] (WB, ICC/IF, IP, Human)	WB, ICC/IF, IP	Human
Wakeman, TP et al. The ATM-SMC1 pathway is essential for activation of the chromium[VI]-induced S-phase checkpoint. Mut Res 554(1-2): 241-251. 2004-01-01 [PMID: 15450422]
Takahashi, TS et al. Recruitment of Xenopus Scc2 cohesin to chromatin requires the pre-replication complex. Nat Cell Biol 6(10): 991-996. 2004-01-01 [PMID: 15448702]
Delia D, Piane M, Buscemi G et al. MRE11 mutations and impaired ATM-dependent responses in an Italian family with ataxia-telangiectasia-like disorder. Hum Mol Genet 2004-09-01 [PMID: 15269180]
Garg, R et al. Chromatin association of Rad17 is requierd for an ataxia telangiectasia Rad-Related kinase-mediated S-phase checkpoint in response to low-dose ultraviolet radiation. Mol Cancer Res 2(6): 362-369. 2004-01-01 [PMID: 15235112] (IP, WB, Human)	IP, WB	Human
Mochan TA, Venere M, DiTullio RA Jr et al. 53BP1 and NFBD1/MDC1-Nbs1 function in parallel interacting pathways activating ataxia-telangiectasia mutated (ATM) in response to DNA damage. Cancer Res 2003-12-01 [PMID: 14695167] (WB, Human)	WB	Human
Wang Y, Qin J. MSH2 and ATR form a signaling module and regulate two branches of the damage response to DNA methylation. Proc Natl Acad Sci U S A 2003-12-01 [PMID: 14657349] (IP, Human)	IP	Human
d'Adda di Fagagna F, Reaper PM, Clay-Farrace L et al. A DNA damage checkpoint response in telomere-initiated senescence. Nature 2003-11-01 [PMID: 14608368]
Udayakumar,D et al. Distinct Pathways of Nonhomologous End Joining That Are Differentially Regulated by DNA-dependent Protein Kinase-mediated Phosphorylation. Journal of Biological Chemistry 278(43):41631-41635. 2003-01-01 [PMID: 12917393]
Musio, A et al. Inhibition of BUB1 results in genomic instability anchorage-independent growth of normal human fibroblasts. Cancer Res 63(11): 2855. 2003-01-01 [PMID: 12782591] (IP, WB, Human)	IP, WB	Human
Goldberg M, Stucki M, Falck J et al. MDC1 is required for the intra-S-phase DNA damage checkpoint. Nature 2003-02-01 [PMID: 12607003]
Yazdi PT, Wang Y, Zhao S et al. SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint. Genes Dev 2002-03-01 [PMID: 11877377] (IP, WB, Human)	IP, WB	Human
Show All 36 Publications. Collapse Publications.