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SMC1L2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: SMC1L2 Antibody [NBP1-89966] - Analysis in human testis and tonsil tissues. Corresponding SMC1B RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: SMC1L2 Antibody [NBP1-89966] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SMC1L2 Antibody [NBP1-89966] - Staining of human tonsil shows no positivity in lymphoid cells.
Immunohistochemistry-Paraffin: SMC1L2 Antibody [NBP1-89966] - Staining of human rectum shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: SMC1L2 Antibody [NBP1-89966] - Staining of human cervix, uterine shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: SMC1L2 Antibody [NBP1-89966] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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SMC1L2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KSTMQILSVQAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLAVDREVGKLQKEVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAESTQATIDIYEKEEAFEIDYSSL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SMC1L2 Protein (NBP1-89966PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SMC1L2 Antibody

  • bK268H5
  • bK268H5.5
  • FLJ43748
  • mitosis-specific chromosome segregation protein like protein beta
  • SMC protein 1B
  • SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
  • SMC1 structural maintenance of chromosomes 1-like 2 (yeast)
  • SMC1 structural maintenance of chromosomes 1-like 2
  • SMC-1B
  • SMC1BETA
  • SMC-1-beta
  • SMC1L2
  • structural maintenance of chromosomes 1B
  • structural maintenance of chromosomes protein 1B

Background

SMC1L2 - SMC1 structural maintenance of chromosomes 1-like 2 (yeast)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMC1B
Entrez
Uniprot