![Immunocytochemistry/Immunofluorescence: Rad21 Antibody [NBP2-20053] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad21 stained by Rad21 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20053-img0016.jpg "Immunocytochemistry/Immunofluorescence: Rad21 Antibody [NBP2-20053] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad21 stained by Rad21 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.")
| Reactivity | Hu, Mu, Rt, Bv, Ch, Pm, RM, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Rad21. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Xenopus (90%), Chicken (94%), Rhesus Macaque (100%), Bovine (98%), Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAD21 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Chip-Assay dependent |
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| Theoretical MW | 72 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Human | 04/11/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Rad21 Antibody (NBP2-20053)Find related products by research area.
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![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0015.jpg "Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - C2C12 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0003.jpg "Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - C2C12 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - HBL435 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0004.jpg "Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - HBL435 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Paraffin-embedded human lung papillary adenocarcinoma. RAD21 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0006.jpg "Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Paraffin-embedded human lung papillary adenocarcinoma. RAD21 antibody diluted at 1:250.")
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Mouse testis. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0014.jpg "Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Mouse testis. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunoprecipitation: Rad21 Antibody [NBP2-20053] - Jurkat whole cell extracts using 5 ug of RAD21 antibody. Western blot analysis was performed using RAD21 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunoprecipitation-NBP2-20053-img0010.jpg "Immunoprecipitation: Rad21 Antibody [NBP2-20053] - Jurkat whole cell extracts using 5 ug of RAD21 antibody. Western blot analysis was performed using RAD21 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20053_rabbit-polyclonal-rad21-antibody-91020242091440.jpg "Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20053_rabbit-polyclonal-rad21-antibody-91020242063390.jpg "Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")