Separase Antibody (6H6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase, but also cleaves itself, promoting downregulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers. Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC-P.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using H00009700-M01	Applications	Species
Hiba O, Cameron G, Mathieu M et al. Mitogen-Induced Defective Mitosis Transforms Neural Progenitor Cells. Neuro Oncol. 2023-04-26 [PMID: 37186014]
H V, K K, T A et al. Varying outcomes of triple-negative breast cancer in different age groups-prognostic value of clinical features and proliferation. Breast Cancer Res Treat. 2022-10-19 [PMID: 36261751]
C Thomas, B Wetherall, MD Levasseur, RJ Harris, ST Kerridge, JMG Higgins, OR Davies, S Madgwick A prometaphase mechanism of securin destruction is essential for meiotic progression in mouse oocytes Nature Communications, 2021-07-14;12(1):4322. 2021-07-14 [PMID: 34262048]
Claudia G, Elena A, Maria U et al. Centrosome Reduction in Newly-Generated Tetraploid Cancer Cells Obtained by Separase Depletion. Sci Rep. 2020-06-04 [PMID: 32499568]
Vihervuori H, Autere T, Repo H et al. Tumor-infiltrating lymphocytes and CD8+ T cells predict survival of triple-negative breast cancer. J Cancer Res Clin Oncol. 2019-09-27 [PMID: 31562550]
Gurvits N, Loyttyniemi E, Nykanen M et al. Separase is a marker for prognosis and mitotic activity in breast cancer. Br. J. Cancer. 2017-10-24 [PMID: 28859055] (IF/IHC, ICC/IF, Human)	IF/IHC, ICC/IF	Human
Mukherjee M, Byrd T, Brawley VS et al. Overexpression and constitutive nuclear localization of cohesin protease Separase protein correlates with high incidence of relapse and reduced overall survival in glioblastoma multiforme. J Neurooncol. 2014-05-04 [PMID: 24792645]
Zhang N, Scorsone K, Ge G et al. Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy. J Biomol Screen. 2014-02-19 [PMID: 24525869]
Mukherjee M, Ge G, Zhang N et al. MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ERalpha)-positive mammary adenocarcinomas. Oncogene. 2013-11-25 [PMID: 24276237]
Panigrahi AK, Zhang N, Mao Q, Pati D. Calpain-1 Cleaves Rad21 to Promote Sister Chromatid Separation. Mol Cell Biol. 2011-08-29 [PMID: 21876002]
Mukherjee M, Ge G, Zhang N et al. Separase Loss of Function Cooperates with the Loss of p53 in the Initiation and Progression of T- and B-Cell Lymphoma, Leukemia and Aneuploidy in Mice. PLoS One. 2011-07-25 [PMID: 21799785]
Zhang N, Ge G, Meyer R et al. Overexpression of Separase induces aneuploidy mammary tumorigenesis. Proc Natl Acad Sci U S A 105(35):13033-8. 2008-09-02 [PMID: 18728194]
Repo H SECURIN-RELATED CELL PROLIFERATION IN BREAST CANCER PROGNOSIS Thesis 2020-01-01 (IHC-P, Human)	IHC-P	Human
Meyer R, Fofanov V, Panigrahi A et al. Overexpression Mislocalization of the Chromosomal Segregation Protein Separase in Multiple Human Cancers. Clin Cancer Res;15(8):2703-10. 2009-04-15 [PMID: 19351757]
Show All 14 Publications. Collapse Publications.

Array H00009700-M01

• Separase Antibodies
• Separase Proteins