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S1P3/EDG-3 Antibody (776807)

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S1P3/EDG-3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human S1P3/EDG-3 Monoclonal Antibody (Catalog # MAB75241) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the marginal zone of germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
S1P3/EDG-3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human S1P3/EDG-3 Monoclonal Antibody (Catalog # MAB75241) at 15 µg/mL overnight at 4 °C. Before incubation with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
776807
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB75241-SP
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S1P3/EDG-3 Antibody (776807) Summary

Immunogen
NS0 mouse myeloma cell line transfected with human S1P3/EDG‑3
Accession # Q99500
Specificity
Detects human S1P3/EDG‑3 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
S1PR3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P3/EDG-3 Antibody (776807)

  • EDG3
  • EDG-3
  • EDG3FLJ37523
  • Endothelial differentiation G-protein coupled receptor 3
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 3
  • FLJ93220
  • G protein-coupled receptor, endothelial differentiation gene-3
  • LPB3
  • MGC71696
  • S1P receptor 3
  • S1P receptor EDG3
  • S1P receptor Edg-3
  • S1P3
  • S1PR3
  • sphingosine 1-phosphate receptor 3
  • Sphingosine 1-phosphate receptor Edg-3
  • sphingosine-1-phosphate receptor 3

Background

EDG-3, also known as sphingosine 1-phosphate receptor-3 (S1P3), is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). EDG-3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human EDG-3 show 74% aa identity with mouse and rat EDG-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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S1P3/EDG-3
Antibody (776807)
MAB75241
S1P3/EDG-3 Antibody (776807)
Species: Hu
Applications: IHC

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Bioinformatics

Gene Symbol S1PR3
Uniprot