Home » LPAR2/LPA2/EDG-4 » LPA2 Antibodies » LPAR2/LPA2/EDG-4 Antibody
Novus Biologicals products are now on bio-techne.com

LPAR2/LPA2/EDG-4 Antibody

 (2 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human LPAR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LPAR2 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human LPAR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6396) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6396-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

LPAR2/LPA2/EDG-4 Antibody Summary

Immunogen
E. coli-derived recombinant human LPAR2
Met1-Val33, His91-Gly103, Ser168-Ser188, Asp264-Tyr279
Accession # Q9HBW0
Specificity
Detects human LPAR2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LPAR1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
LPAR2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using AF6396.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LPAR2/LPA2/EDG-4 Antibody

  • EDG4
  • EDG-4
  • EDG4FLJ93869
  • endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor4,LPA receptor EDG4
  • G protein-coupled receptor
  • LPA2
  • LPA-2
  • LPA2LPA receptor 2
  • LPAR2
  • lysophosphatidic acid receptor 2
  • lysophosphatidic acid receptor EDG4
  • Lysophosphatidic acid receptor Edg-4

Background

LPAR2 (Lysophosphatidic acid receptor 2; also LPA2 and EDG4) is a 39 kDa member of the GPCR1 family of proteins.  It has limited expression, and is found on mammalian macrophages, fibroblasts and cardiac myocytes.  LPAR2 binds to, and is activated by LPA, a molecule principally derived from activated platelets.  Although LPAR2 appears to prefer myristoyl and palmitoyl LPA, multiple forms of LPA are recognized by the receptor.  LPAR2 reportedly mediates chemotaxis, arachidonic acid release, and cell rounding.  Human LPAR2 is a 351 amino acid (aa) 7–transmembrane (TM) protein (SwissProt # Q9HBW0).  It contains a 33 aa extracellular N-terminus (aa #1-33), seven TM segments (aa #34-297) and a 54 aa C-terminal cytoplasmic tail.  There is one splice variant that is associated with tumor cells, and which originally was given the designation EDG4 (endothelium differentiation gene 4).  This shows a 35 aa substitution for aa #348-351.  Over aa #1-33, 91-103, 168-188 and 264-279 collectively, human LPAR2 shares 75% aa identity with mouse LPAR2.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

LPAR1/LPA1/EDG-2
Antibody - BSA Free
NBP1-03363
Simple Western LPAR1/LPA1/EDG-2 Antibody - BSA Free
Western Blot LPAR1/LPA1/EDG-2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence LPAR1/LPA1/EDG-2 Antibody - BSA Free
(1 Review)
(7 Publications)
Species: Ch, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Lipoprotein a
Antibody (4H1) - BSA
NBP2-37477
ELISA Lipoprotein a Antibody (4H1) - BSA Free
Western Blot Lipoprotein a Antibody (4H1) - BSA Free
Immunocytochemistry/ Immunofluorescence Lipoprotein a Antibody (4H1) - BSA Free
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
LPAR3/LPA3/EDG-7
Antibody - BSA Free
NLS1017
Immunohistochemistry-Paraffin LPAR3/LPA3/EDG-7 Antibody - BSA Free
Immunohistochemistry-Paraffin LPAR3/LPA3/EDG-7 Antibody - BSA Free
Immunohistochemistry-Paraffin LPAR3/LPA3/EDG-7 Antibody - BSA Free
Species: Bt, Bv, Ca, Eq, Hu, Mu
Applications: IHC, IHC-P
BUD31 Antibody
NBP1-84010
Western Blot BUD31 Antibody
Immunohistochemistry-Paraffin BUD31 Antibody
Immunocytochemistry/ Immunofluorescence BUD31 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
LPAR4/LPA4
Antibody - BSA Free
NLS1895
Immunohistochemistry-Paraffin LPAR4/LPA4 Antibody - BSA Free
Immunohistochemistry-Paraffin LPAR4/LPA4 Antibody - BSA Free
Immunohistochemistry-Paraffin LPAR4/LPA4 Antibody - BSA Free
Species: Bt, Ha, Hu, Pm, Mu, Pm, Rt
Applications: Flow, IHC, IHC-P
S1P1/EDG-1
Antibody (713412) [Un ...
MAB7089
Flow Cytometry S1P1/EDG-1 Antibody (713412) [Unconjugated]
Flow Cytometry S1P1/EDG-1 Antibody (713412) [Unconjugated]
Immunohistochemistry S1P1/EDG-1 Antibody (713412) [Unconjugated]
(28 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(62 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Recombinant Human
MBTPS1 GST (N-Term) P ...
H00008720-Q01
Other Recombinant Human MBTPS1 GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, WB
S1P2/EDG-5/S1PR2
Antibody
NBP2-26691
Immunocytochemistry/ Immunofluorescence S1P2/EDG-5/S1PR2 Antibody
Western Blot S1P2/EDG-5/S1PR2 Antibody
(1 Review)
(8 Publications)
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
S1P3/EDG-3 Antibody
NBP2-24762
Western Blot S1P3/EDG-3 Antibody
Immunohistochemistry-Paraffin S1P3/EDG-3 Antibody
Western Blot S1P3/EDG-3 Antibody
Species: Hu, Mu
Applications: IHC, IHC-P, WB
ENPP-2/Autotaxin
[Unconjugated]
5255-EN
ENPP-2/Autotaxin [Unconjugated]
(5 Publications)
Species: Hu
Applications: EnzAct
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(50 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(183 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(685 Publications)
Species: Hu
Applications: ELISA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(665 Publications)
Species: Mu
Applications: ELISA
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(848 Publications)
Species: Hu
Applications: BA
LPAR2/LPA2/EDG-4
Antibody
AF6396
LPAR2/LPA2/EDG-4 Antibody
Species: Hu
Applications: WB

Publications for LPAR2/LPA2/EDG-4 Antibody (AF6396)(2)


Reviews for LPAR2/LPA2/EDG-4 Antibody (AF6396) (0)

There are no reviews for LPAR2/LPA2/EDG-4 Antibody (AF6396). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LPAR2/LPA2/EDG-4 Antibody (AF6396) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional LPAR2/LPA2/EDG-4 Products

Blogs on LPAR2/LPA2/EDG-4

There are no specific blogs for LPAR2/LPA2/EDG-4, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LPAR2/LPA2/EDG-4 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol LPAR2
Uniprot