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S1P2/EDG-5/S1PR2 Antibody

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Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and ...read more
Immunocytochemistry/ Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Human PC3 prostate cancer cell line. Primary antibody NBP2-26691 at 1:200, secondary antibody Alexa488, Hoechst 33342 used for nuclear ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Po, XpSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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S1P2/EDG-5/S1PR2 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 325-343 of human S1P2/Edg5 was used as immunogen, GenBank no. NP_004221.3.
Predicted Species
Porcine (94%), Xenopus (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S1PR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 25621765)
  • Western Blot 1-2 ug/ml
Reviewed Applications
Read 1 Review rated 4
using
NBP2-26691 in the following applications:

Publications
Read Publications using
NBP2-26691 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S1P2/EDG-5/S1PR2 Antibody

  • AGR16
  • EDG5
  • EDG-5
  • endothelial differentiation G-protein coupled receptor 5
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 5
  • GPCR13
  • GPCR18
  • H218
  • LPB2
  • S1P receptor 2
  • S1P receptor EDG5
  • S1P receptor Edg-5
  • S1P2
  • S1PR2
  • sphingosine 1-phosphate receptor 2
  • sphingosine 1-phosphate receptor Edg-5
  • sphingosine-1-phosphate receptor 2

Background

This EDG5 protein is a member of the G protein-coupled receptors, as well as the EDG family of proteins. This protein participates in sphingosine 1-phosphate-induced cell proliferation, survival, and transcriptional activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.


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ICC/IF
(2)
IF/IHC
(2)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Rat
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP2-26691 Applications Species
Jiang H, Huang T, Yu Y et al. Characterization of a S1PR2 specific 11C-labeled radiotracer in streptozotocin-induced diabetic murine model Nuclear Medicine and Biology 2023-07-01 [PMID: 37556928] (IHC-Fr, Mouse) IHC-Fr Mouse
Xue J, Lin J, Liu Z et al. Alleviating early demyelination in ischaemia/reperfusion by inhibiting sphingosine-1-phosphate receptor 2 could protect visual function from impairment Brain pathology (Zurich, Switzerland) 2023-05-04 [PMID: 37142391] (IHC, WB, Mouse) IHC, WB Mouse
Janneh AH, Kassir MF, Atilgan FC et al. Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis Cell Reports 2022-12-06 [PMID: 36476873]
Xiao P, Gu J, Xu W et al. RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction Autophagy 2022-03-09 [PMID: 35263212] (IF/IHC, Rat) IF/IHC Rat
Pan G, Liao M, Dai Y et al. Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture Frontiers in Cardiovascular Medicine 2021-12-17 [PMID: 34977175] (ICC/IF, IF/IHC, WB, Human) ICC/IF, IF/IHC, WB Human
Tran HB, Maiolo S, Harper R Et al. Dysregulated zinc and sphingosine-1-phosphate signaling in pulmonary hypertension: Potential effects by targeting of bone morphogenetic protein receptor type 2 in pulmonary microvessels Cell biology international 2021-08-04 [PMID: 34347342] (ICC/IF, Rat) ICC/IF Rat
Gu Y, Shea J, Slattum G et al. Defective apical extrusion signaling contributes to aggressive tumor hallmarks Elife. 2015-02-11 [PMID: 25621765] (IHC-P, Human)

Details:
S1P2/EDG-5/S1PR2 antibody used for IHC-P staining of formalin-fixed, paraffin-embedded tissue sections of Human Pancreatic Ductal Adenocarcinomas /PDACs - heat induced antigen retrieval with 10 mM sodium citrate for 20 minutes, blocking with 5% BSA/0.5% Tween-20 in PBS for 4 hours, primary incubated for ON at 4C, detection with Alexa-568 anti-rabbit secondary antibody (Figure 9A).
IHC-P Human
Kempf A, Tews B, Arzt ME et al. The sphingolipid receptor S1PR2 is a receptor for Nogo-a repressing synaptic plasticity. PLoS Biol. 2014-01-01 [PMID: 24453941] (WB, Mouse) WB Mouse

Review for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-26691:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence S1P2/EDG-5/S1PR2 NBP2-26691
Enlarge
4
reviewed by:
Dimitra Kalamida
IF Human 07/11/2018
View

Summary

ApplicationImmunofluorescence
Sample TestedC42B prostate cell line,PC3 prostate cancer cell line
SpeciesHuman
Lot01013168A-04

Comments

Comments1:200 dilution of the antibody used

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Dimitra Kalamida
07/11/2018
Application: IF
Species: Human

Bioinformatics

Gene Symbol S1PR2
Uniprot