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RUNX1/CBFA2 Antibody [Unconjugated]

 
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Western blot shows cytoplasmic and nuclear extracts from Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human RUNX1/CBFA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows cytoplasmic and nuclear extracts from Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for RUNX1/CBFA2 at approximately 59 kDa (as indicated) using 20 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2399
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2399-SP
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RUNX1/CBFA2 Antibody [Unconjugated] Summary

Specificity
Detects human RUNX1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RUNX1
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Applications/Dilutions

Dilutions
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RUNX1/CBFA2 Antibody [Unconjugated]

  • Acute myeloid leukemia 1 protein
  • AML1
  • AML1acute myeloid leukemia 1
  • AML1-EVI-1
  • CBFA2
  • CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1
  • Core-binding factor subunit alpha-2
  • EVI-1
  • PEBP2A2
  • runt domain, alpha subunit 2
  • runt-related transcription factor 1
  • RUNX1
  • SL3/AKV core-binding factor alpha B subunit

Background

RUNX1, also known as CBFA2, is one of three members of the Runt-related family. The RUNX/CBFA proteins form the alpha  subunits that combine with the beta subunit (CBFB) to form the heterodimeric transcription factors. RUNX/CBFA plays important roles in hematopoiesis and may regulate the generation of hematopoietic stem cells from hematogenic endothelial cells. Deregulated expression of CBFA2/RUNX1 is associated with malignant diseases of the hematopoietic system. Human and mouse RUNX/CBFA share 95% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RUNX1
Uniprot